Distinct migratory behavior of early-and late-born neurons derived from the cortical ventricular zone
Distinct migratory behavior of early-and late-born neurons derived from the cortical ventricular zone
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DOI：
10.1002/cne.20256
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发表时间：
2004-11-01
影响因子：
2.5
通讯作者：
Murakami, F
中科院分区：
文献类型：
--
作者：
Hatanaka, Y;Hisanaga, SI;Murakami, F
延时研究表明，脑室区（VZ）来源的细胞在哺乳动物胚胎发生的不同阶段在大脑皮层表现出两种迁移模式：染色体易位和运动。我们进行了系统的分析，以检查皮层神经元的迁移行为是否来源于皮层VZ阶段依赖。用绿色荧光蛋白（gfp）编码质粒在子宫内电穿孔法标记小鼠胚胎VZ细胞，并在适当的存活时间后对标记细胞进行评价。在胚胎期(E) 12.5或E15.5电穿孔后，GFP+ VZ细胞最初呈纺锤状，呈放射状定向。离开VZ后，它们转变为圆形或水平方向的梭状神经元，具有许多短突起。当它们向上移动时，它们似乎逐渐转变为径向定向的双极细胞。在E12.5标记的VZ细胞（早期出生的神经元）最早迁移到皮层板（CP）的顶部，而E15.5标记的VZ细胞（晚期出生的神经元）进一步沿着径向纤维的长度迁移到皮层板的顶部。然后将细胞周期蛋白依赖性激酶5 （Cdk5DN）基因的显性阴性形式引入VZ细胞。用cdk5dn转染E12.5 VZ未破坏早期出生神经元的迁移。然而，这导致晚出生的神经元迁移失败，尽管它们在中间区域转化为双极形状。因此，皮层神经元似乎至少有两个不同的迁移阶段：一个是早期和晚期出生的神经元共同的，另一个是晚期出生的神经元和依赖cdk5的特异性迁移阶段。(C) 2004 Wiley-Liss, Inc。
Time-lapse studies indicate that ventricular zone (VZ)-derived cells show two migratory modes in the cerebral cortex at different stages of mammalian embryogenesis: somal translocation and locomotion. We carried out a systematic analysis to examine whether the migratory behavior of cortical neurons derived from the cortical VZ is stage-dependent. We labeled VZ cells of mouse embryos with green fluorescent protein (gfp)-encoding plasmids by in utero electroporation and evaluated the labeled cells after appropriate survival periods. After electroporation at either embryonic day (E) 12.5 or E15.5, GFP+ VZ cells were initially spindle-shaped and radially oriented. After leaving the VZ, they transformed into round or horizontally oriented fusiform neurons with many short processes. They then seemed to gradually change into radially oriented bipolar cells as they moved upward. Whereas the earliest emigrants from the VZ labeled at E12.5 (early-born neurons) reached the top of the cortical plate (CP) after these changes, VZ cells labeled at E15.5 (late-born neurons) further migrated along the length of radial fibers to reach the top of the CP. A dominant negative form of the gene for cyclin-dependent kinase 5 (Cdk5DN) was then introduced into VZ cells. Transfection of E12.5 VZ with cdk5dn did not disrupt the migration of the early-born neurons. However, this caused a failure in migration of the late-born neurons, although they transformed into bipolar shapes in the intermediate zone. Thus, there appear to be at least two distinct migratory phases of cortical neurons: one common to the early- and late-born neurons, and the other specific to late-born neurons and Cdk5-dependent. (C) 2004 Wiley-Liss, Inc.