Conditioned medium of the primary culture of rat choroid plexus epithelial (modified ependymal) cells enhances neurite outgrowth and survival of hippocampal neurons
Conditioned medium of the primary culture of rat choroid plexus epithelial (modified ependymal) cells enhances neurite outgrowth and survival of hippocampal neurons
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DOI：
10.1016/j.neulet.2004.12.068
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发表时间：
2005-05
影响因子：
2.5
通讯作者：
Yumi Watanabe;N. Matsumoto;M. Dezawa;Yutaka Itokazu;T. Yoshihara;C. Idé
中科院分区：
文献类型：
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作者：
Yumi Watanabe;N. Matsumoto;M. Dezawa;Yutaka Itokazu;T. Yoshihara;C. Idé
脉络丛上皮(修饰的室管膜)细胞(CPECs)专用于产生脑脊液(CSF)，是血-脑脊液屏障。提示CPECs除产生脑脊液外，还可能通过向脑脊液中表达分泌因子来调节中枢神经系统功能。有报道称，中巴经济走廊表达各种类型的因素，包括生长因子。然而，在体内和体外，CPECs产生和分泌的分子对中枢神经系统(CNS)的实际影响几乎是未知的。利用CPECs的纯培养，我们证明CPECs的条件培养液(CM)能促进新生1d大鼠海马神经元培养24小时的轴突生长和存活。在天然条件下，超滤法将CM的作用保留在含有大于50 kDa分子络合物的组分中，经胰酶消化后，CM的作用消失。本研究的结果表明，CPECs可以通过分泌可能是多肽/蛋白质性质的因子而不是小的化学物质来支持体外培养的神经元的生存和功能。
The choroid plexus epithelial (modified ependymal) cells (CPECs) are specialized for cerebrospinal fluid (CSF) production and serve as blood–CSF barrier. It is suggested that, in addition to CSF production, the CPECs may regulate CNS function through expression of secretory factors into CSF. There have been reports that the CPECs express various types of factors including growth factors. However, the actual effects of the molecules produced and secreted from the CPECs on the central nervous system (CNS) are virtually unknown both in vivo and in vitro. With the use of pure culture of CPECs, we demonstrated that the conditioned medium (CM) from CPECs can enhance neurite outgrowth and survival of cultured neurons derived from rat hippocampus on postnatal day 1 in 24-h cultures. The effect of the CM was retained in fractions that contains complex of molecules larger than 50kDa in native condition with ultrafiltration method and disappeared by trypsin digestion. The results of the present study indicate that CPECs can support the survival and function of neurons in vitro by secreting factors that are likely to be of peptide/protein nature rather than small chemicals.