Similar effects of osmolarity, glucose, and phosphate on cleavage past the 2-cell stage in mouse embryos from outbred and F1 hybrid females
Similar effects of osmolarity, glucose, and phosphate on cleavage past the 2-cell stage in mouse embryos from outbred and F1 hybrid females
复制标题
DOI：
10.1095/biolreprod.104.033324
复制
发表时间：
2005-01-01
影响因子：
3.6
通讯作者：
Baltz, JM
中科院分区：
文献类型：
--
作者：
Hadi, T;Hammer, MA;Baltz, JM
来自大多数随机繁殖和近交系雌性小鼠的单细胞期胚胎在经典胚胎培养基中的两细胞期表现出体外发育阻滞。然而，在相同条件下，来自许多杂种F1的胚很容易发育过两细胞期。这引起了普遍接受的观点，即存在阻断型和非阻断型雌性小鼠，只有前者倾向于两细胞阻断。最近，培养介质已经改进到这样的程度，即使是倾向于两细胞块的胚胎也会在体外发育，从而使研究其病因成为可能。在这里，我们表明，无论是增加渗透压或增加葡萄糖/磷酸盐水平诱导预期的两个细胞块在随机繁殖的CF 1胚胎和两个细胞块在增加渗透压可以获救的有机渗透压剂甘氨酸。令人惊讶的是，来自B6 D2 F（1）（BDF 1）F-1杂交雌性的单细胞胚胎，被认为是非阻断的，当渗透压或葡萄糖/磷酸盐水平增加时，在两细胞阶段也被阻断。它们也同样被甘氨酸从渗透压诱导的阻滞中解救出来。最明显的区别是，据称非阻塞胚胎成为阻塞在一个更高的阈值渗透压或葡萄糖/磷酸盐水平比那些被认为容易出现这种发育阻滞。因此，阻断和非阻断胚胎实际上都表现出类似的两细胞发育阻滞。
One-cell-stage embryos derived from most random-bred and inbred female mice exhibit an in vitro developmental block at the two-cell stage in classical embryo culture media. However, embryos derived from many F, hybrids develop easily past the two-cell stage under the same conditions. This has given rise to the commonly accepted idea that there exist blocking and non-blocking types of female mice, with only the former being prone to a two-cell block. Recently, culture media have been improved to the point that even embryos prone to the two-cell block will develop past the block in vitro, making it possible to study its etiology. Here, we show that either increased osmolarity or increased glucose/phosphate levels induced the expected two-cell block in random-bred CF1 embryos and the two-cell block at increased osmolarities could be rescued by the organic osmolyte glycine. Surprisingly, one-cell embryos from B6D2F(1) (BDF1) F-1 hybrid females, considered to be nonblocking, also became blocked at the two-cell stage when osmolarity or glucose/phosphate levels were increased. They were also similarly rescued by glycine from the osmolarity-induced block. The most evident difference was that the purportedly nonblocking embryos became blocked at a higher threshold of osmolarity or glucose/phosphate level than those considered prone to this developmental block. Thus, both blocking and nonblocking embryos actually exhibit a similar two-cell block to development.