Towards a comprehensive atlas of cortical connections in a primate brain: Mapping tracer injection studies of the common marmoset into a reference digital template.
Towards a comprehensive atlas of cortical connections in a primate brain: Mapping tracer injection studies of the common marmoset into a reference digital template.
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迈向灵长类动物大脑中皮质连接的全面地图集：将公共摩尔马斯群岛的示踪剂注射研究映射到参考数字模板中。
DOI：
10.1002/cne.24023
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发表时间：
2016-08-01
期刊：
影响因子：
--
通讯作者：
Rosa MG
中科院分区：
文献类型：
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作者：
Majka P;Chaplin TA;Yu HH;Tolpygo A;Mitra PP;Wójcik DK;Rosa MG
狨猴是一种新兴的动物模型，用于大规模地了解灵长类动物的大脑连接，但要实现这一目标，需要开发和验证许多神经解剖学实验结果的规范化和整合程序。在这里，我们描述了一个计算管道，用于将皮层区域连接的逆行跟踪数据共配到3D狨猴大脑模板中，该模板由尼氏染色切片生成。该过程产生一系列空间变换，这些变换应用于不同情况下标记神经元的坐标，将它们带入共同的立体空间。作为早期研究的一部分，我们在9只狨猴的额叶中进行了17次注射。这些注射所显示的皮层连接模式的可视化作为补充资料提供。对这些病例的自动化和人工处理结果的比较表明，注射部位的中心可以重建，平均而言，经验丰富的神经解剖学家估计的坐标误差在0.6毫米以内。此外，通过自动化方法在不同区域获得的细胞计数与专家获得的细胞计数高度相关（r = 0.83），专家详细检查了每个个体的组织学切片。目前的程序能够对大型数据集进行比较和可视化，这反过来又为整合和分析来自许多动物的结果开辟了道路。它的多功能性，包括对档案材料的适用性，可能会减少产生灵长类动物大脑皮层连接体的第一个细节所需的额外实验的数量。[j] .中国生物医学工程学报，2016。©2016作者比较神经病学杂志由Wiley期刊公司出版。
The marmoset is an emerging animal model for large‐scale attempts to understand primate brain connectivity, but achieving this aim requires the development and validation of procedures for normalization and integration of results from many neuroanatomical experiments. Here we describe a computational pipeline for coregistration of retrograde tracing data on connections of cortical areas into a 3D marmoset brain template, generated from Nissl‐stained sections. The procedure results in a series of spatial transformations that are applied to the coordinates of labeled neurons in the different cases, bringing them into common stereotaxic space. We applied this procedure to 17 injections, placed in the frontal lobe of nine marmosets as part of earlier studies. Visualizations of cortical patterns of connections revealed by these injections are supplied as Supplementary Materials. Comparison between the results of the automated and human‐based processing of these cases reveals that the centers of injection sites can be reconstructed, on average, to within 0.6 mm of coordinates estimated by an experienced neuroanatomist. Moreover, cell counts obtained in different areas by the automated approach are highly correlated (r = 0.83) with those obtained by an expert, who examined in detail histological sections for each individual. The present procedure enables comparison and visualization of large datasets, which in turn opens the way for integration and analysis of results from many animals. Its versatility, including applicability to archival materials, may reduce the number of additional experiments required to produce the first detailed cortical connectome of a primate brain. J. Comp. Neurol. 524:2161–2181, 2016. © 2016 The Authors The Journal of Comparative Neurology Published by Wiley Periodicals, Inc.