IDENTIFICATION AND CHARACTERIZATION OF 2 CAMPYLOBACTER-JEJUNI ADHESINS FOR CELLULAR AND MUCOUS SUBSTRATES
IDENTIFICATION AND CHARACTERIZATION OF 2 CAMPYLOBACTER-JEJUNI ADHESINS FOR CELLULAR AND MUCOUS SUBSTRATES
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DOI：
10.1128/iai.53.1.141-148.1986
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发表时间：
1986-07-01
影响因子：
3.1
通讯作者：
WALKER, RI
中科院分区：
文献类型：
--
作者：
MCSWEEGAN, E;WALKER, RI
空肠弯曲杆菌能够定殖于人类肠粘膜并引起疾病。因此，研究C.空肠粘附于上皮细胞和肠粘液凝胶。所有菌株均为C.使用的空肠能够粘附INT 407上皮细胞和粘液，但对一种基质的高粘附性不一定表明对另一种基质的粘附性相当。C.通过用蛋白酶或戊二醛处理细菌细胞或通过向培养基中添加某种碳水化合物（岩藻糖或甘露糖）来部分抑制空肠对细胞的作用。C.通过比较鞭毛化和无鞭毛化变体的粘附，将空肠鉴定为潜在的粘附素。剪切的细菌细胞，以消除鞭毛减少细菌的粘附，而固定的鞭毛与KCN增加粘附。纯化的鞭毛表现出特定的，岩藻糖耐药结合上皮细胞，但不肠粘液。第二，非蛋白质粘附素的存在下，建议，因为没有一个单一的治疗细菌完全抑制粘附。脂多糖（LPS）被鉴定为另一种C。空肠粘附素[3H]LPS特异性结合上皮细胞，这种现象被LPS的高碘酸盐氧化或上皮细胞的戊二醛固定所抑制。与鞭毛不同，LPS是岩藻糖敏感的，并且抑制整个细菌细胞与INT 407细胞的结合。LPS也能够结合肠粘液凝胶。这些数据表明鞭毛和LPS在粘附于粘膜表面方面都是重要的。
Campylobacter jejuni is able to colonize the human intestinal mucosa and cause disease. For this reason, it was important to investigate mechanisms by which C. jejuni adheres to epithelial cells and intestinal mucus gel. All strains of C. jejuni used were able to adhere to INT 407 epithelial cells and mucus, but high adherence to one substrate did not necessarily indicate comparable adherence to the other. The adherence of C. jejuni to cells was inhibited partially by treating the bacterial cells with proteases or glutaraldehyde or by adding a certain carbohydrate (fucose or mannose) to the medium. The flagellum of C. jejuni was identified as a potential adhesin by comparing adherence of flagellated and aflagellated variants. Shearing of the bacterial cells to remove the flagella reduced bacterial adhesion, whereas immobilization of the flagellum with KCN increased adhesion. Purified flagella showed specific, fucose-resistant binding to epithelial cells but not to intestinal mucus. The presence of a second, nonproteinaceous adhesin was suggested because no single treatment of the bacteria completely inhibited adhesion. Lipopolysaccharide (LPS) was identified as another C. jejuni adhesin. [3H]LPS specifically bound to epithelial cells, and this phenomenon was inhibited by periodate oxidation of the LPS or glutaraldehyde fixation of the epithelial cells. LPS, unlike flagella, was fucose sensitive and inhibited binding of whole bacterial cells to INT 407 cells. LPS was also able to bind to intestinal mucus gel. These data indicate that both flagella and LPS are important in adhesion to the mucosal surface.