The Trypanosoma cruzi genome; conserved core genes and extremely variable surface molecule families
The Trypanosoma cruzi genome; conserved core genes and extremely variable surface molecule families
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DOI：
10.1016/j.resmic.2011.05.003
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发表时间：
2011-07-01
影响因子：
2.6
通讯作者：
Andersson, Bjorn
中科院分区：
文献类型：
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作者：
Andersson, Bjorn
原生动物寄生虫克氏锥虫是一种重要但被忽视的病原体，它会导致恰加斯病，影响数百万人，主要是在拉丁美洲。这种寄生虫的种群结构和流行病学很复杂，不同菌株之间有很大的差异。参考菌株CL Brener的基因组序列于2005年公布，该序列的出现不仅揭示了寄生虫基因组的复杂性，而且通过提供8000多个核心基因的序列，极大地促进了寄生虫生物学和发病机制的研究。T CRUZI基因组高度重复，这导致了基因组序列的不准确，人们试图对重复基因进行更深入的分析，作为基因组序列的补充。基因组被发现组织在包含家务和其他基因的稳定核心区，周围是高度重复的、通常是亚端粒的高度可变区，包含表面分子基因大家族的多个成员。对T ruzi株进行了比较测序，结果表明，该寄生虫的核心基因内容高度保守，但存在很大的序列变异性，编码区不同菌株之间的DNA水平平均差异3-4%。额外的基因组将提高对寄生虫生物学和流行病学的理解。(C)2011年巴斯德研究所。爱思唯尔·马森公司出版。版权所有。
The protozoan parasite Trypanosoma cruzi is an important but neglected pathogen that causes chagas disease, which affects millions of people, mainly in latin America. The population structure and epidemiology of the parasite are complex, with much variability among strains. The genome sequence of a reference strain, CL Brener, was published in 2005, and the availability of this sequence has both revealed the complexity of the parasite genome and greatly facilitated research into parasite biology and pathogenesis, by making the sequences of more than 8000 core genes available. The T cruzi genome is highly repetitive, which has resulted in inaccuracies in the genome sequence, and attempts have been made to provide a deeper analysis of repeated genes as a complement to the genome sequence. The genome was found to be organized in stable core regions containing housekeeping and other genes, surrounded by highly repetitive, often sub-telomeric highly variable regions containing multiple members of large families of surface molecule genes. Comparative sequencing of T cruzi strains has been initiated and the results show that the core gene content of the parasite is highly conserved, but that much sequence variability, 3-4% difference at the DNA level on average between strains in coding regions, is present. The additional genomes will improve the understanding of parasite biology and epidemiology. (c) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.