Synthesis and characterization of dendron cross-linked PEG hydrogels as corneal adhesives.
Synthesis and characterization of dendron cross-linked PEG hydrogels as corneal adhesives.
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DOI：
10.1021/bm200039s
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发表时间：
2011-05-09
影响因子：
6.2
通讯作者：
Grinstaff MW
中科院分区：
文献类型：
--
作者：
Oelker AM;Berlin JA;Wathier M;Grinstaff MW
为了寻求伤口特异性角膜粘合剂，对丙醛、丁醛或琥珀酸 2-氧乙基官能化聚乙二醇 (PEG) 与基于肽的树突状交联剂 (Lys3Cys4) 反应形成的水凝胶进行了表征。这些大分子单体在混合后几分钟内发生反应，形成透明且有弹性的水凝胶，其体外降解时间根据交联反应过程中形成的键类型（噻唑烷或假脯氨酸）从数小时到数月不等。通过平行板流变学测定的这些材料的机械性能取决于聚合物浓度，水凝胶粘合强度也是如此，水凝胶粘合强度是通过搭接剪切粘合测试测定的。此外，这些水凝胶可有效闭合离体4.1毫米中央角膜撕裂伤：用这些水凝胶粘合剂闭合的伤口能够承受相当于或超过缝合闭合伤口所获得的眼内压值。
In pursuit of a wound-specific corneal adhesive, hydrogels formed by the reaction of propionaldehyde, butyraldehyde, or 2-oxoethyl succinate-functionalized poly(ethylene glycol) (PEG) with a peptide-based dendritic cross-linker (Lys3Cys4) were characterized. These macromers react within minutes of mixing to form transparent and elastic hydrogels with in vitro degradation times that range from hours to months based on the type of bonds formed during the cross-linking reaction – either thiazolidine or pseudoproline. The mechanical properties of these materials, determined via parallel plate rheology, were dependent upon the polymer concentration, as was the hydrogel adhesive strength, which was determined by lap shear adhesive testing. In addition, these hydrogels were efficacious in closing ex vivo 4.1 mm central corneal lacerations: wounds closed with these hydrogel adhesives were able to withstand intraocular pressure values equivalent to, or in excess of, those obtained by closing the wounds with suturing.