Gene amplifications in osteosarcoma -: CGH microarray analysis
Gene amplifications in osteosarcoma -: CGH microarray analysis
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DOI：
10.1002/gcc.20120
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发表时间：
2005-02-01
影响因子：
3.7
通讯作者：
Knuutila, S
中科院分区：
文献类型：
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作者：
Atiye, J;Wolf, M;Knuutila, S
对于骨肉瘤的基因组改变知之甚少。我们在包含约13,000个基因克隆的cDNA微阵列上使用比较基因组杂交技术对22个骨肉瘤样本进行了全基因组高分辨率基因拷贝数分析。22例中有19例的扩增片段平均长度超过1mb，包含10多个基因。确定了许多增益和损失区域，并以高分辨率定义了它们的边界。在14q11、17q25和22q11-q13位点发现了新的扩增子。在22个样本中，分别有8个、6个、5个和4个样本在12q11-q15、8q24、6p12-p13和17p1-p13处检测到先前已知的大扩增区域。12q扩增比以前报道的更频繁（36%的病例）。先前已知的1p34-p36, 1q21, 19q13和21q22的小扩增在至少三个病例中被发现。我们的研究结果表明，TOM1L2和CYP27B1分别是17p和12q扩增子的新靶点。每个扩增子中扩增基因的详细信息（www.helsinki.fi/cmg）为进一步的硅研究提供了有价值的原始数据。(C) 2004 Wiley-Liss, Inc。
Little is known about the genomic alterations underlying osteosarcoma. We performed a genomewide high-resolution gene copy number analysis of 22 osteosarcoma samples using comparative genomic hybridization on a cDNA microarray that contained cDNA clones of about 13,000 genes. Nineteen of the 22 cases had amplifications that on average spanned more than 1 Mb and contained more than 10 genes. Numerous regions of gain and loss were identified, and their boundaries were defined at high resolution. Novel amplicons were found at 14q11, 17q25, and 22q11-q13. Earlier-known large amplified regions were detected at 12q11-q15, 8q24, 6p12-p13, and 17p1-p13 in 8, 6, 5, and 4 of the 22 samples, respectively. Amplification of 12q was observed more frequently (36% of the cases) than previously reported. Previously known small amplicons at 1p34-p36, 1q21, 19q13, and 21q22 were seen in at least three cases. Our results implicate TOM1L2 and CYP27B1 as having roles as novel targets for the 17p and 12q amplicons, respectively. Details (www.helsinki.fi/cmg) of the amplified genes in each amplicon provide valuable raw data for further in silico studies. (C) 2004 Wiley-Liss, Inc.