Ratiometric porphyrin-based layers and nanoparticles for measuring oxygen in biosamples
Ratiometric porphyrin-based layers and nanoparticles for measuring oxygen in biosamples
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DOI：
10.1016/j.snb.2008.09.039
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发表时间：
2009-01-15
影响因子：
8.4
通讯作者：
Mohr, Gerhard J.
中科院分区：
文献类型：
--
作者：
Cywinski, Piotr J.;Moro, Artur J.;Mohr, Gerhard J.
氧敏聚苯乙烯层和纳米颗粒已被开发用于测量生物样品中的氧。聚合物层通过旋涂在玻璃板上制备，而纳米颗粒通过微乳液聚合合成。铂（II）meso-四（五氟苯基）卟啉（PtTFPP）用作氧传感器，而N，N '-双（1-己基庚基）二萘嵌苯-3，4：9，10-双（二甲酰亚胺）（S13）用作参比染料。对于层和纳米颗粒两者，在水溶液中以及在酵母培养物中评估传感器响应。观察到氧对卟啉磷光的猝灭，而参比染料的荧光基本上保持不受影响。通过使用参考染料的信号，可以容易地校正激发光强度或纳米传感器浓度的波动。这两种传感器系统进行了比较，他们的光谱响应和灵敏度。(c)2008 Elsevier B. V.保留所有权利。
Oxygen-sensitive polystyrene layers and nanoparticles have been developed for measuring oxygen in biosamples. Polymer layers were prepared via spin-coating on glass plates while the nanoparticles were synthesized by microemulsion polymerization. Platinum(II)meso-tetra(pentafluorophenyl)porphine (PtTFPP) was used as an oxygen sensor whereas N,N'-bis(1-hexylheptyl)perylene-3,4:9,10-bis(dicarboximide) (S13) was used as a reference dye. For both, layers and nanoparticles, the sensor response was assessed in aqueous solution as well as in yeast culture. Quenching of porphyrin phosphorescence by oxygen was observed whereas fluorescence of the reference dye remained essentially unaffected. By using the signal of the reference dye fluctuations in the intensity of the excitation light or the nanosensor concentration can be easily corrected. Both sensor systems were compared with respect to their spectral response and their sensitivity. (c) 2008 Elsevier B.V. All rights reserved.