Effect of repeated adrenocorticotropic hormone administration on reproductive function and hair cortisol concentration during the estrous cycle in goats
Effect of repeated adrenocorticotropic hormone administration on reproductive function and hair cortisol concentration during the estrous cycle in goats
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DOI：
10.1016/j.ygcen.2017.11.027
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发表时间：
2018-04-01
影响因子：
2.7
通讯作者：
Tanaka, Tomomi
中科院分区：
文献类型：
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作者：
Endo, Natsumi;Yamane, Hiroaki;Tanaka, Tomomi
头发中皮质醇浓度的测量已被用作包括人类、野生动物和家畜在内的多个物种的慢性应激指标。然而，头发中的皮质醇浓度如何准确地反映循环皮质醇浓度的变化尚未得到充分记录。本研究的目的是检查反复施用促肾上腺皮质激素（ACTH）对山羊发情周期生殖功能和毛发皮质醇浓度的影响。在实验1中，山羊在发情周期的第11-17天（第0天是排卵日）每天一次肌内注射ACTH（0.625 IU/10 kg体重，n = 6）或生理盐水（n = 6），持续7天。在实验2中，在发情周期的第11-24天，每天两次向山羊肌肉注射ACTH（0.625 IU/10 kg体重，n = 6）或盐水（n = 6）。首次给药后 0、0.5 和 6 小时采集血样以确定循环皮质醇浓度。在开始给药后0、1和2个月剪掉头发。在这两个实验中，血浆皮质醇浓度在 ACTH 给药后 0.5 小时增加，并在 6 小时恢复到基线。在实验期间，在ACTH组和盐水组中的大多数动物中观察到发情（实验1中分别为6/6和4/6，实验2中分别为5/6和6/6），并且在所有检查的山羊中观察到排卵。然而，ACTH 组和盐水组之间的排卵卵泡数量存在显着差异，并且 ACTH 组和盐水组之间的排卵卵泡最大直径也存在差异（P =.07）。在实验1中，在整个采样期间，头发皮质醇浓度不受ACTH施用的影响。实验2中，ACTH组毛发皮质醇浓度在给药后1个月时高于给药前值，但在2个月时没有显着差异。这些结果表明，重复给予ACTH会影响卵泡的发育和排卵过程，并且毛发皮质醇浓度的分析可用于评估循环中皮质醇浓度的相对长期变化。 (C) 2017 Elsevier Inc. 保留所有权利。
Measurement of the cortisol concentration in hair has been used as an index of chronic stress in several species including humans, wildlife and domestic animals. However, how accurately the cortisol concentration in hair reflects the changes in circulating cortisol concentrations has not been well documented. The objective of the present study was to examine the effect of repeated adrenocorticotropic hormone (ACTH) administration on the reproductive function during the estrous cycle and hair cortisol concentrations in goats. In experiment 1, goats were administered ACTH (0.625 IU/10 kg of body weight, n = 6) or saline (n = 6) intramuscularly once a day for 7 days on Day 11-17 of the estrous cycle (day 0 was the day of ovulation). In experiment 2, goats were administered ACTH (0.625 IU/10 kg of body weight, n = 6) or saline (n = 6) intramuscularly twice a day on Day 11-24 of the estrous cycle. Blood samples were collected 0, 0.5, and 6 h after first administration to determine the circulating cortisol concentrations. Hair was clipped at 0, 1, and 2 months after the start of administration. In both experiments, the plasma cortisol concentration increased at 0.5 h and returned to baseline at 6 h after ACTH administration. During the experiments, estrus was observed in most animals in ACTH and saline groups (6/6 and 4/6 in experiment 1 and 5/6 and 6/6 in experiment 2, respectively) and ovulation was observed in all goats examined. However, the number of ovulatory follicles was significantly different between the ACTH and saline groups, and the maximal diameter of ovulatory follicles tended to be different (P =.07) between the ACTH and saline groups. In experiment 1, the hair cortisol concentration was not influenced by the ACTH administration throughout the sampling period. In experiment 2, the hair cortisol concentration in the ACTH group was greater at 1 month after administration than the pre-administration value, but was not significantly different at 2 months. These results suggest that repeated ACTH administration affects the development and ovulatory process of ovarian follicles and analysis of the hair cortisol concentration can be used for assessing relatively long-term changes in cortisol concentration in the circulation. (C) 2017 Elsevier Inc. All rights reserved.