In vitro study on the individual and synergistic cytotoxicity of adriamycin and selenium nanoparticles against Bel7402 cells with a quartz crystal microbalance.
In vitro study on the individual and synergistic cytotoxicity of adriamycin and selenium nanoparticles against Bel7402 cells with a quartz crystal microbalance.
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DOI：
10.1016/j.bios.2008.10.030
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发表时间：
2009-03
影响因子：
12.6
通讯作者：
Liang Tan;Xue'en Jia;Xiangfu Jiang;Youyu Zhang;Hao Tang;S. Yao;Q. Xie
中科院分区：
文献类型：
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作者：
Liang Tan;Xue'en Jia;Xiangfu Jiang;Youyu Zhang;Hao Tang;S. Yao;Q. Xie
以海藻酸钠（SA）为模板，通过还原亚硒酸（H2SeO3）制备硒纳米粒子（Se NPs）。应用石英晶体微天平（QCM）实时监测药物诱导人肝癌细胞Bel7402凋亡的过程。研究了阿霉素（ADM）与硒纳米粒联合使用的抗肿瘤作用。结果发现，这两种药物都能够以剂量依赖性的方式抑制细胞增殖，并且与ADM和Se NPs的组合治疗在抑制细胞生长方面比单独使用两种药物中的每一种更有效。根据Δ f0反应，采用改良的Bürgi公式（Jin公式）评价药物联合的细胞毒效应。随着药物作用时间的延长，药物的协同作用逐渐由明显的协同作用变为简单的相加作用，但较低浓度的药物联合作用在24 h后仍表现出协同作用，提示其在肿瘤治疗中具有潜在的应用价值。
Selenium nanoparticles (Se NPs) were prepared based on the reduction of selenious acid (H2SeO3), by employing sodium alginate (SA) as a template. The real-time monitoring of the drug-inducing apoptosis process of human hepatic cancer cells Bel7402 was performed with the quartz crystal microbalance (QCM) measurement. The anti-tumor effect of adriamycin (ADM) used in combination with Se NPs was investigated. It is found that both drugs were able to inhibit cell proliferation in a dose-dependent way and the combined treatment with ADM and Se NPs was more effective in inhibiting cell growth than each of the two drugs alone. The cytotoxic effects of drug combination were evaluated with the modified Bürgi formula (Jin equation) based the Δf0responses. The grades gradually changed from apparent synergism to simple addition with the drug-treatment time increasing but the drug combination with lower concentrations still exhibited synergism after 24h, suggesting a potential application in cancer therapy.