FLUORESCENCE DEPOLARIZATION BY ELECTRONIC-ENERGY TRANSFER IN DONOR-ACCEPTOR PAIRS OF LIKE AND UNLIKE CHROMOPHORES
FLUORESCENCE DEPOLARIZATION BY ELECTRONIC-ENERGY TRANSFER IN DONOR-ACCEPTOR PAIRS OF LIKE AND UNLIKE CHROMOPHORES
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DOI：
10.1063/1.461285
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发表时间：
1991-12-01
影响因子：
4.4
通讯作者：
VALEUR, B
中科院分区：
文献类型：
--
作者：
BERBERANSANTOS, MN;VALEUR, B
研究了在固定距离和随机不相关的静态方向上，供体-受体对中，由偶极-偶极相互作用（福斯特型）引起的能量转移引起的荧光去极化。 对于不同的发色团，受体各向异性衰减显示三种不同的极端类型的行为。 当受体的固有衰减速率比转移速率和供体固有衰减速率都快得多时，受体各向异性从正值衰减，然后上升并通过最大值，最后趋于负极限值，产生零稳态值。 最大值的存在是由于取向因子和由施主和受主跃迁矩形成的平均角之间的特殊关系。 对于类似的发色团对，精确的各向异性计算和比较，由近似处理。 它还表明，间接激发的合作伙伴的各向异性随时间而变化，趋于零，在以前的工作，据报道，它是4%的直接激发的发色团的矛盾。
Fluorescence depolarization by energy transfer resulting from dipole-dipole interaction (Forster type) is studied in donor-acceptor pairs of like and unlike chromophores at a fixed distance and with random and uncorrelated static orientations. For unlike chromophores, the acceptor anisotropy decay is shown to display three different extreme types of behavior. When the intrinsic decay rate of the acceptor is much faster than both the transfer rate and the donor intrinsic decay rate, the acceptor anisotropy decays from a positive value, then rises and passes through a maximum, and finally tends to a negative limiting value, yielding a zero steady-state value. The existence of a maximum is shown to be due to the peculiar relation between the orientation factor and the average angle formed by the donor and acceptor transition moments. For pairs of like chromophores, the exact anisotropy is calculated and compared with that given by an approximate treatment. It is also shown that the anisotropy of the indirectly excited partner varies with time, tending to zero, in contradiction to previous work, where it is reported to be 4% of that of the directly excited chromophore.