Translation control:: bridging the gap between genomics and proteomics?
Translation control:: bridging the gap between genomics and proteomics?
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DOI：
10.1016/s0968-0004(00)01776-x
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发表时间：
2001-04-01
影响因子：
13.8
通讯作者：
Garcia-Sanz, JA
中科院分区：
文献类型：
--
作者：
Pradet-Balade, B;Boulmé, F;Garcia-Sanz, JA
mRNA谱能够同时监测细胞中数千种转录物的表达水平。然而，在酵母和哺乳动物细胞中的分析表明，单独的mRNA水平是相应蛋白质丰度的不可靠指标。mRNA和蛋白质水平之间的这种差异证明了除了转录之外的其他控制机制的相关性。由于翻译控制是调节基因表达的主要机制，在分析实验中使用翻译的mRNA可能比使用总mRNA更接近地描述蛋白质组。这将联合收割机结合基因组学的技术潜力与蛋白质组学的生理相关性。
mRNA profiling enables the expression levels of thousands of transcripts in a cell to be monitored simultaneously. Nevertheless, analyses in yeast and mammalian cells have demonstrated that mRNA levels alone are unreliable indicators of the corresponding protein abundances. This discrepancy between mRNA and protein levels argues for the relevance of additional control mechanisms besides transcription. As translational control is a major mechanism regulating gene expression, the use of translated mRNA in profil in g experiments might depict the proteome more closely than does the use of total mRNA. This would combine the technical potential of genomics with the physiological relevance of proteomics.