DISTINCT MOLECULAR-FORMS OF HUMAN T-CELL RECEPTOR GAMMA-DELTA DETECTED ON VIABLE T-CELLS BY A MONOCLONAL-ANTIBODY
DISTINCT MOLECULAR-FORMS OF HUMAN T-CELL RECEPTOR GAMMA-DELTA DETECTED ON VIABLE T-CELLS BY A MONOCLONAL-ANTIBODY
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DOI：
10.1084/jem.167.5.1625
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发表时间：
1988-05-01
影响因子：
15.3
通讯作者：
VANDEGRIEND, RJ
中科院分区：
文献类型：
--
作者：
BORST, J;VANDONGEN, JJM;VANDEGRIEND, RJ
第二种类型的TCR分子已经在人类和小鼠的T淋巴细胞上被发现，它涉及伽马射线的蛋白质产物。和.德尔塔。基因。T淋巴细胞，这涉及到伽马的蛋白质产物。和.德尔塔。基因。携带该受体的T淋巴细胞可能构成具有不同免疫功能的独立细胞谱系。我们已经生产了一种mAb，它能特异性地检测人类TCR-γ/β。在自然和变性状态下，这与以前使用的反-伽马形成对比。链状多肽血清，仅与变性蛋白反应。该受体以不同的分子形式存在，有或没有链间二硫键，其中一个。通过细胞表面碘化可以检测到链，也可以不检测到链。这种单抗与所有这些受体形式都有反应。因此，该抗体可用于测定TCR-γ/β的表达。在存活的人类T淋巴细胞上。在正常人中，TCR-伽马/增量。外周血中CD3+淋巴细胞占2~7%，胸腺中占0.1%~1.0%。这些细胞中的大多数不表达CD4或CD8抗原，尽管有相当比例的CD8+细胞被发现。根据超微结构分析判断，外周血中的TCR-γ/β+细胞为静息淋巴细胞。具有不同受体类型的T细胞克隆可以表现出MHC非限制性细胞杀伤活性，这被证明是由培养条件诱导的，很可能是由IL-2等生长因子诱导的。这强烈地表明，TCR-伽马/增量。在MHC非限制性细胞毒性中不起到靶细胞识别的作用。反TCR-伽马/增量。抗体可以在表达受体的克隆中特异性地诱导细胞毒活性，但另外还能抑制生长因子诱导的细胞毒作用，这表明TCR-γ/β的调节作用。CD3复合体在MHC中的非限制性细胞毒作用。
A second type of TCR molecule has been identified on human and murine T lymphocytes, which involves the protein products of the .gamma. and .delta. genes. T lymphocytes, which involves the protein products of the .gamma. and .delta. genes. T lymphocytes bearing this receptor may constitute a separate cell lineage with a distinct immune function. We have produced an mAb, which specifically detects human TCR-.gamma./.delta. in native as well as denatured states, this in contrast to previously used anti-.gamma. chain peptide sera, which only reacted with denatured protein. The receptor occurs in different molecular forms, with or without interchain disulphide bonds, in which a .delta. chain may or may not be detected by cell surface iodination. This mAb is reactive with all these receptor forms. Therefore, this antibody could be used to determine the expression of TCR-.gamma./.delta. on viable human T lymphocytes. In normal individuals, TCR-.gamma./.delta. was found on a subset composing 2-7% of CD3+ lymphocytes in peripheral blood and 0.1-1.0% in thymus. The majority of these cells do not express the CD4 or CD8 antigens, although a significant percent of CD8+ cells was found. TCR-.gamma./.delta.+ cells in peripheral blood are resting lymphocytes, as judged by ultrastructural analysis. T cell clones with different receptor types can display MHC-nonrestricted cytolytic activity, which is shown to be induced by the culture conditions, most likely by growth factors such as IL-2. This strongly suggests that TCR-.gamma./.delta. does not play a role in target cell recognition in MHC-nonrestricted cytotoxicity. The anti-TCR-.gamma./.delta. antibody can specifically induce cytotoxic activity in clones expressing the receptor, but in addition inhibit growth factor induced cytotoxicity, which indicates a regulatory role of the TCR-.gamma./.delta. CD3 complex in MHC-nonrestricted cytotoxicity.