13C natural abundance in serum retinol acts as a biomarker for increases in dietary provitamin A.
13C natural abundance in serum retinol acts as a biomarker for increases in dietary provitamin A.
复制标题
13c血清视黄醇中的天然丰度充当饮食中蛋白蛋白A的生物标志物。
DOI：
10.3181/0806-rm-199
复制
发表时间：
2009-02
期刊：
影响因子：
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通讯作者：
Tanumihardjo SA
中科院分区：
文献类型：
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作者：
Howe JA;Valentine AR;Hull AK;Tanumihardjo SA
组织中13C和12C的天然同位素组成在很大程度上取决于饮食。维生素A原类胡萝卜素的来源（如蔬菜）通常比来自玉米饲料动物的预制维生素A来源（如乳制品和肉类）具有更低的13C与12C比率（13C:12C），这在美国很普遍。在一项为期3个月的饮食干预中，研究人员评估了血清视黄醇的13C:12C （13C:12C-视黄醇）作为蔬菜摄入量的生物标志物，该干预旨在通过增加蔬菜摄入量或减少卡路里和脂肪摄入量来促进体重减轻。受试者年龄21-50岁，BMI在30-40 kg/m2之间，来自美国的一个地理区域。高蔬菜组（n = 20）被鼓励将每天的蔬菜和水果摄入量增加到0.95升蔬菜和0.24-0.35升水果。热量减少组（n = 17）被鼓励每天减少500千卡的热量摄入，并从脂肪中摄入不超过25%的热量。每天提供75-100%的蔬菜和水果目标以及50-67%的卡路里和脂肪克。在研究期间，受试者停止补充类胡萝卜素。血清视黄醇和维生素A原类胡萝卜素浓度；摄入预先形成的维生素A、维生素A原和脂肪；并分析了体重、脂肪质量和瘦质量与13C: 12c视黄醇的相关性。干预后蔬菜组13C: 12c -视黄醇含量下降（P = 0.050），与维生素A原摄入量的相关性接近显著（P = 0.079）。13C: 12c -视黄醇在热量减少组中没有变化（P = 0.43）。13C: 12c -视黄醇随着饮食中维生素A来源的变化而变化，可以作为饮食中维生素A原蔬菜摄入量增加的生物标志物。
The natural isotopic composition of 13C and 12C in tissues is largely determined by the diet. Sources of provitamin A carotenoids (e.g., vegetables) typically have a lower 13C to 12C ratio (13C:12C) than preformed vitamin A sources (i.e., dairy and meat) from corn-fed animals, which are prevalent in the US. The 13C:12C of serum retinol (13C:12C-retinol) was evaluated as a biomarker for vegetable intake in a 3-mo dietary intervention designed to promote weight-loss by increased vegetable consumption or reduced calorie and fat intake. Subjects were 21–50 y of age with a BMI between 30–40 kg/m2 and were enrolled from one geographic area in the US. The high vegetable group (n = 20) was encouraged to increase daily vegetable and fruit consumption to 0.95 liter vegetables and 0.24–0.35 liter fruits. The caloric reduction group (n = 17) was encouraged to lower caloric intake by 500 kcal and consume ≤25% kcal from fat daily. Provided meals supplied 75–100% vegetable and fruit goals and 50–67% kcal and fat g per day. Carotenoid supplementation was discontinued by subjects during the study. Serum retinol and provitamin A carotenoid concentrations; intake of preformed vitamin A, provitamin A, and fat; and body weight, fat mass, and lean mass were analyzed for correlations to 13C:12C-retinol. 13C:12C-Retinol decreased in the vegetable group after intervention (P = 0.050) and the correlation with provitamin A intake was approaching significance (P = 0.079). 13C:12C-Retinol did not change in the caloric reduction group (P = 0.43). 13C:12C-Retinol changes with the vitamin A source in the diet and can be used as a biomarker for increases in dietary provitamin A vegetable intake.