Aptamer-conjugated Mn3O4@SiO2 core-shell nanoprobes for targeted magnetic resonance imaging
Aptamer-conjugated Mn3O4@SiO2 core-shell nanoprobes for targeted magnetic resonance imaging
复制标题
用于靶向磁共振成像的适配体共轭 Mn3O4@SiO2 核壳纳米探针。
DOI：
10.1039/c3nr03490a
复制
发表时间：
2013-01-01
期刊：
影响因子：
6.7
通讯作者：
Yang, Shiping
中科院分区：
文献类型：
--
作者：
Hu, He;Dai, Antao;Yang, Shiping
本研究的目的是评估适配体 (AS411) 缀合 Mn3O4@SiO2 核壳纳米探针 (NP) 在人宫颈癌荷瘤小鼠体内的靶向 T1 磁共振成像 (MRI)、定量生物分布和毒性。首先通过将疏水性 Mn3O4 核封装在氨基功能化的二氧化硅壳内来制备纳米颗粒。将荧光团罗丹明（RB）掺杂到二氧化硅壳中，并在壳表面修饰两亲性聚合物聚乙二醇（PEG）以提高其生物相容性，然后将适体AS411缀合到PEG链末端作为靶向配体。最终的纳米粒子缩写为Mn3O4@SiO2(RB)-PEG-Apt。通过体外荧光共聚焦成像和体内MRI，纳米颗粒已被证明可以有效地靶向癌细胞和显着的肿瘤聚集。定量生物分布研究进一步证实了成像结果。此外，组织学、血液学和生物化学分析也证明了纳米粒子在体内的低毒性。我们的结果表明，Mn3O4@SiO2(RB)-PEG-Apt NPs 具有巨大的潜力，可以用作癌症长期靶向成像和治疗的多功能纳米平台。
The objective of this study was to evaluate the targeted T1-magnetic resonance imaging (MRI), quantitative biodistribution and toxicity of aptamer (AS411) conjugated Mn3O4@SiO2 core-shell nanoprobes (NPs) in human cervical carcinoma tumor-bearing mice. The NPs were firstly prepared by encapsulating a hydrophobic Mn3O4 core within an amino functionalized silica shell. The fluorophore rhodamine (RB) was doped into the silica shell and the amphiphilic polymer poly(ethylene glycol) (PEG) was modified on the surface of the shell to improve its biocompatibility, then the aptamer AS411 was conjugated onto the end of the PEG chains as targeting ligands. The final NPs were abbreviated as Mn3O4@SiO2(RB)-PEG-Apt. By means of in vitro fluorescence confocal imaging and in vivo MRI, the NPs have been demonstrated to target cancer cells and prominent tumor aggregation effectively. The imaging results were further confirmed by a quantitative biodistribution study. In addition, histological, hematological and biochemistry analysis also proved the low toxicity of NPs in vivo. Our results showed the great potential of the Mn3O4@SiO2(RB)-PEG-Apt NPs could be used as a multifunctional nanoplatform for long-term targeted imaging and therapy of cancer.