Novel synthesis and characterization of bioconjugate block copolymers having oligonucleotides.
Novel synthesis and characterization of bioconjugate block copolymers having oligonucleotides.
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DOI：
10.1021/bm0502462
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发表时间：
2005-06
影响因子：
6.2
通讯作者：
Atsushi Noro;Yutaka Nagata;M. Tsukamoto;Y. Hayakawa;A. Takano;Y. Matsushita
中科院分区：
文献类型：
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作者：
Atsushi Noro;Yutaka Nagata;M. Tsukamoto;Y. Hayakawa;A. Takano;Y. Matsushita
已经开发出通过亚磷酰胺方法新型且有效地合成以核苷酸为末端的聚合物。羟基封端的聚合物分三步转化为核苷酸封端的聚合物，其中反应的转化率非常高，几乎100%。通过重复该合成方法，成功合成了由合成聚合物、聚苯乙烯和具有胸苷单元的生物寡核苷酸组成的嵌段共聚物。通过透射电子显微镜和小角X射线散射观察该嵌段共聚物的微相分离结构，确认了圆柱结构。
Novel and efficient synthesis of polymers terminated with nucleotides via the phosphoramidite method has been developed. A hydroxyl-terminated polymer was converted into a polymer capped with a nucleotide in three steps, where the conversion of the reactions was very high, almost 100%. By repetition of this synthetic method, a block copolymer composed of a synthetic polymer, polystyrene, and biological oligonucleotides with thymidine units has been successfully synthesized. A microphase-separated structure of this block copolymer was observed by both transmission electron microscopy and small-angle X-ray scattering, and a cylindrical structure was confirmed.