Multidrug-resistance gene (mdr1) expression in human tumor xenografts.
Multidrug-resistance gene (mdr1) expression in human tumor xenografts.
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人类肿瘤异种移植物中的多药耐药基因（mdr1）表达。
DOI：
10.3892/ijo.5.6.1285
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发表时间：
1994
影响因子：
5.2
通讯作者：
N. Tamaoka
中科院分区：
文献类型：
--
作者：
Y. Abe;M. Nakamura;Y. Ohnishi;M. Inaba;Y. Ueyama;N. Tamaoka
我们检测了人多药耐药基因（MDR 1）的表达，34个异种移植物中有13个通过逆转录-聚合酶链反应显示出不同的MDR 1基因转录水平。MDR 1在10例肺癌、8例胃癌、3例结肠癌和3例胰腺癌中分别为3例、5例、3例和2例，而在4例乳腺癌和6例胶质瘤中均未检测到。我们评估了体内异种移植物的药物敏感性; 34个异种移植物中有28个对长春新碱和/或多柔比星表现出耐药性，而28个耐药异种移植物中有13个表达MDR 1。MDR 1的表达可以解释某些异种移植物的内在耐药性。
We examined the expression of human multidrug resistance gene (MDR1); 13 of 34 xenografts showed various MDR1 gene transcript levels by reverse transcriptase-polymerase chain reaction. MDR1 was detected in 3 of 10 lung, 5 of 8 gastric, 3 of 3 colon and 2 of 3 pancreatic cancers, but not in 4 breast cancers, or 6 gliomas. We evaluated drug-sensitivity of xenografts in vivo; 28 of 34 xenografts showed drug resistance to vincristine and/or doxorubicin, whereas 13 of 28 drug-resistant xenografts expressed MDR1. The expression of MDR1 may explain the intrinsic drug resistance in certain xenografts.