Pol-miR-363-3p plays a significant role in the immune defense of Japanese flounder Paralichthys olivaceus against bacterial and viral infection.
Pol-miR-363-3p plays a significant role in the immune defense of Japanese flounder Paralichthys olivaceus against bacterial and viral infection.
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DOI：
10.1016/j.fsi.2020.06.016
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发表时间：
2020-06
影响因子：
4.7
通讯作者：
Yan-ling Sun;Xiao-lu Guan;Peng Zhang;Mo-fei Li;Jian Zhang;Li Sun
中科院分区：
文献类型：
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作者：
Yan-ling Sun;Xiao-lu Guan;Peng Zhang;Mo-fei Li;Jian Zhang;Li Sun
在这项研究中，我们检测了一种日本比目鱼（Paralichthys olivaceus）的microRNA (miRNA) pol-miR-363-3p的功能。我们发现pol-miR-363-3p靶向泛素特异性蛋白酶（USP） USP32。USP是一个去泛素酶家族，对泛素蛋白酶体系统的功能至关重要。在哺乳动物中，USP32已知与癌症和免疫有关。在鱼类中，USP32的功能尚不清楚。我们发现，在比目鱼的主要组织中，尤其是肠道中都检测到USP32 （PoUSP32）的表达。体外研究表明，pol-miR-363-3p通过与PoUSP32的3'UTR相互作用，以负向方式直接调控PoUSP32。在牙鲆细胞中过表达pol-miR-363-3p或干扰PoUSP32表达可显著阻断链球菌感染。同样，在体内敲低pol-miR-363-3p或过表达PoUSP32可增强s的传播。在比目鱼组织中，psp32的体内敲低抑制了eds。iniaedissemination。此外，pol-miR-363-3p敲低还显著促进了病毒病原体巨噬细胞病毒的组织传播，巨噬细胞病毒和as . iniae调节了pol-miR-363-3p的表达。这些结果揭示了pol-miR-363-3p在牙鲆免疫防御细菌和病毒感染中的重要作用。
In this study, we examined the function of a Japanese flounder (Paralichthys olivaceus) microRNA (miRNA), pol-miR-363-3p. We found that pol-miR-363-3p targets an ubiquitin-specific protease (USP), USP32. USP is a family of deubiquitinating enzymes essential to the functioning of the ubiquitin proteasome system. In mammals, USP32 is known to be associated with cancer and immunity. In fish, the function of USP32 is unknown. We found that flounder USP32 (PoUSP32) expression was detected in the major tissues of flounder, particularly intestine.In vitroandin vivostudies showed that pol-miR-363-3p directly regulated PoUSP32 in a negative manner by interaction with the 3′UTR of PoUSP32. Overexpression of pol-miR-363-3p or interference with PoUSP32 expression in flounder cells significantly blockedStreptococcus iniaeinfection. Consistently,in vivoknockdown of pol-miR-363-3p or overexpression of PoUSP32 enhanced dissemination ofS. iniaein flounder tissues, whereasin vivoknockdown of PoUSP32 inhibitedS. iniaedissemination. In addition, pol-miR-363-3p knockdown also significantly promoted the tissue dissemination of the viral pathogen megalocytivirus, which, as well asS. iniae, regulated pol-miR-363-3p expression. Together these results revealed an important role of pol-miR-363-3p in flounder immune defense against bacterial and viral infection.