Analysis and manipulation of hematopoietic progenitor and stem cells from murine embryonic tissues.
Analysis and manipulation of hematopoietic progenitor and stem cells from murine embryonic tissues.
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DOI：
10.1002/9780470151808.sc02a06s4
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发表时间：
2008
影响因子：
--
通讯作者：
A. Medvinsky;S. Taoudi;Sandra Mendes;E. Dzierzak
中科院分区：
文献类型：
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作者：
A. Medvinsky;S. Taoudi;Sandra Mendes;E. Dzierzak
造血发育始于哺乳动物胚胎的几个位置：卵黄囊、主动脉-性腺-中肾区 (AGM) 和绒毛膜尿囊胎盘。最有效的细胞，即成体定形造血干细胞 (HSC)，发生在妊娠中期小鼠胚胎体内的 AGM 区域。同样，在人类胚胎的同等发育时间，AGM 区域已被证明含有多能祖细胞。因此，小鼠胚胎是研究造血发育的绝佳模型。为了进一步研究成人造血系统的个体发育，本单元的重点是用于分析 AGM 区域的实验方法。
Hematopoietic development begins in several locations in the mammalian embryo: yolk sac, aorta-gonad-mesonephros region (AGM), and the chorio-allantoic placenta. Generation of the most potent cells, adult definitive hematopoietic stem cells (HSCs), occurs within the body of the mouse embryo at midgestation in the AGM region. Similarly, at the equivalent developmental time in the human embryo, the AGM region has been shown to contain multipotent progenitors. Hence, the mouse embryo serves as an excellent model to study hematopoietic development. To further studies on the ontogeny of the adult hematopoietic system, the focus of this unit is on the experimental methods used in analysis of the AGM region.