Salinity tolerance in soybean is modulated by natural variation in GmSALT3
Salinity tolerance in soybean is modulated by natural variation in GmSALT3
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大豆的耐盐性受到 GmSALT3 自然变异的调节
DOI：
10.1111/tpj.12695
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发表时间：
2014-12-01
期刊：
影响因子：
7.2
通讯作者：
Qiu, Lijuan
中科院分区：
文献类型：
--
作者：
Guan, Rongxia;Qu, Yue;Qiu, Lijuan
提高作物耐盐性的基因鉴定是盐碱地有效利用的基础。在这里，我们对大豆（Glycine max (L.)）进行了精细定位。从商品品种铁丰8号和85-140衍生的Merr.)群体中鉴定出3号染色体上的GmSALT3（耐盐相关基因），这一显性基因与限制大豆茎部钠离子（Na+）的积累和大幅提高大豆耐盐性有关。GmSALT3编码一个来自阳离子/H+交换家族的蛋白，该蛋白定位于内质网，在耐盐亲本铁峰8号与韧皮部和木质部相关的根细胞中优先表达。我们在盐敏感亲本85-140中发现，在Gmsalt3的外显子3插入了一个3.78 kb的反转录座子，截断了转录本。通过对31个大豆地方品种和22个野生大豆（甘氨酸大豆）进行测序，共鉴定出9个单倍型，其中2个耐盐单倍型和7个盐敏感单倍型。通过对172个地方大豆品种和57个野生大豆品种的单倍型分布分析，发现铁丰8号的单倍型1 （H1）与耐盐性密切相关，可能是其祖先等位基因。不具有耐盐性的等位基因H2-H6、H8和H9是最近才获得的。与其他等位基因不同，H1具有广泛的地理范围，包括盐碱地，这表明它在需要时得到维持，但在自然选择和驯化过程中，它的强耐受性可能会丧失。GmSALT3是一个与大豆耐盐性相关的基因，具有很大的改良潜力。
The identification of genes that improve the salt tolerance of crops is essential for the effective utilization of saline soils for agriculture. Here, we use fine mapping in a soybean (Glycine max (L.) Merr.) population derived from the commercial cultivars Tiefeng 8 and 85-140 to identify GmSALT3 (salt tolerance-associated gene on chromosome 3), a dominant gene associated with limiting the accumulation of sodium ions (Na+) in shoots and a substantial enhancement in salt tolerance in soybean. GmSALT3 encodes a protein from the cation/H+ exchanger family that we localized to the endoplasmic reticulum and which is preferentially expressed in the salt-tolerant parent Tiefeng 8 within root cells associated with phloem and xylem. We identified in the salt-sensitive parent, 85-140, a 3.78-kb copia retrotransposon insertion in exon 3 of Gmsalt3 that truncates the transcript. By sequencing 31 soybean landraces and 22 wild soybean (Glycine soja) a total of nine haplotypes including two salt-tolerant haplotypes and seven salt-sensitive haplotypes were identified. By analysing the distribution of haplotypes among 172 Chinese soybean landraces and 57 wild soybean we found that haplotype 1 (H1, found in Tiefeng 8) was strongly associated with salt tolerance and is likely to be the ancestral allele. Alleles H2-H6, H8 and H9, which do not confer salinity tolerance, were acquired more recently. H1, unlike other alleles, has a wide geographical range including saline areas, which indicates it is maintained when required but its potent stress tolerance can be lost during natural selection and domestication. GmSALT3 is a gene associated with salt tolerance with great potential for soybean improvement.