Direct cardiac reprogramming: Toward the era of multi-omics analysis.
Direct cardiac reprogramming: Toward the era of multi-omics analysis.
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DOI：
10.1016/j.cellin.2022.100058
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发表时间：
2022-12
期刊：
影响因子：
--
通讯作者：
Wang, Li
中科院分区：
文献类型：
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作者：
Liu, Mengxin;Liu, Jie;Zhang, Tong;Wang, Li
成年心肌细胞有限的再生能力阻碍了心脏损伤后的修复和再生。直接心脏重编程将瘢痕形成的心脏成纤维细胞（CF）转化为功能性诱导心肌细胞（iCM），为恢复心脏结构和心脏功能提供了有希望的潜力。使用遗传和表观遗传调节剂、小分子和递送策略在iCM重编程中已经取得了重大进展。最近的研究异质性和重编程轨迹阐明了新的机制，iCM重编程在单细胞水平。在这里，我们回顾了iCM重编程的最新进展，重点是多组学（转录组学，表观基因组学和蛋白质组学）的研究，以探讨细胞命运转换的细胞和分子机制。我们还强调了未来的潜力，使用多组学方法解剖iCM转换为临床应用。
Limited regenerative capacity of adult cardiomyocytes precludes heart repair and regeneration after cardiac injury. Direct cardiac reprograming that converts scar-forming cardiac fibroblasts (CFs) into functional induced-cardiomyocytes (iCMs) offers promising potential to restore heart structure and heart function. Significant advances have been achieved in iCM reprogramming using genetic and epigenetic regulators, small molecules, and delivery strategies. Recent researches on the heterogeneity and reprogramming trajectories elucidated novel mechanisms of iCM reprogramming at single cell level. Here, we review recent progress in iCM reprogramming with a focus on multi-omics (transcriptomic, epigenomic and proteomic) researches to investigate the cellular and molecular machinery governing cell fate conversion. We also highlight the future potential using multi-omics approaches to dissect iCMs conversion for clinal applications.