Altered expression of circadian clock gene, mPer1, in mouse brain and kidney under morphine dependence and withdrawal.
Altered expression of circadian clock gene, mPer1, in mouse brain and kidney under morphine dependence and withdrawal.
复制标题
在吗啡依赖性和戒断下，小鼠脑和肾脏中昼夜节律基因MPER1的表达改变。
DOI：
10.1186/1740-3391-4-9
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发表时间：
2006-08-22
影响因子：
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通讯作者：
Ding JM
中科院分区：
文献类型：
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作者：
Wang X;Wang Y;Xin H;Liu Y;Wang Y;Zheng H;Jiang Z;Wan C;Wang Z;Ding JM
人体的每项生理功能都表现出某种形式的昼夜节律。然而，在病理条件下，昼夜节律可能会被破坏。感染艾滋病毒或吸毒成瘾的患者经常患有睡眠障碍和昼夜节律改变。果蝇的早期研究表明，药物寻求行为可能与某些生物钟基因的表达有关。我们之前的研究表明，吗啡治疗后的条件性位置偏好在缺乏Period-1 (mPer1) 生物钟基因的小鼠中发生了改变。因此，我们试图研究吗啡治疗是否可以改变 mPer1 的表达，特别是在 SCN 以外的大脑区域和周围组织中。我们使用蛋白质印迹分析的结果表明，mPER1 免疫反应性在对照 (Con)、吗啡依赖 (MD) 和吗啡戒断 (MW) 小鼠的大脑中表现出强烈的昼夜节律。然而，MD小鼠大脑中mPER1表达的昼夜节律相位与Con小鼠显着不同(p < 0.05)。与大脑中的 mPER1 表达相反，给予吗啡后，肾脏中 mPER1 免疫反应性的昼夜节律被消除，而 Con 小鼠则维持了肾脏中 mPER1 的稳健昼夜节律。因此，吗啡对生物钟基因mPer1的影响在不同器官之间可能存在差异，导致SCN与周围器官之间的昼夜节律功能不同步。
Every physiological function in the human body exhibits some form of circadian rhythmicity. Under pathological conditions, however, circadian rhythmicity may be dusrupted. Patients infected with HIV or addicted to drugs of abuse often suffer from sleep disorders and altered circadian rhythms. Early studies in Drosophila suggested that drug seeking behavior might be related to the expression of certain circadian clock genes. Our previous research showed that conditioned place preference with morphine treatment was altered in mice lacking the Period-1 (mPer1) circadian clock gene. Thus, we sought to investigate whether morphine treatment could alter the expression of mPer1, especially in brain regions outside the SCN and in peripheral tissues. Our results using Western blot analysis showed that the mPER1 immunoreactivity exhibited a strong circadian rhythm in the brains of the control (Con), morphine-dependent (MD), and morphine-withdrawal (MW) mice. However, the phase of the circadian rhythm of mPER1 expression in the brains of MD mice significantly differed from that of the Con mice (p < 0.05). In contrast to mPER1 expression in the brain, the circadian rhythm of mPER1 immunoreactivity in the kidneys was abolished after morphine administration, whereas the Con mice maintained robust circadian rhythmicity of mPER1 in the kidney. Therefore, the effect of morphine on the circadian clock gene mPer1 may vary among different organs, resulting in desynchronization of circadian function between the SCN and peripheral organs.