Different functions of QTL for estrogen-dependent tumor growth of the rat pituitary.
Different functions of QTL for estrogen-dependent tumor growth of the rat pituitary.
复制标题
QTL对大鼠垂体雌激素依赖性肿瘤生长的不同功能。
DOI：
10.1007/s003350010168
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Gorski,J
中科院分区：
文献类型：
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作者：
Wendell,DL;Daun,SB;Stratton,MB;Gorski,J
对Fischer 344（F344）品系大鼠进行雌激素治疗可诱导垂体肿瘤的生长，这些肿瘤表现出细胞增殖加速、基底膜破裂和出血湖的形成。雌激素依赖的垂体生长是由于一组数量性状位点（QTL）的变化，称为Edpm雌激素依赖的垂体质量，我们以前确定在F344和抗肿瘤布朗挪威株的F2杂交。我们以前确定了5个QTL，和微卫星标记，因为我们早期的工作，使我们能够扫描新的染色体区域，导致两个新的QTL雌激素依赖性垂体质量：Edpm 9 - 2和一个可能的QTL的X染色体（Chr）。在这里，我们报告的证据表明，这些QTL在如何影响生长方面彼此不同。为了研究EdpmQTL对肿瘤生长生化成分的影响，我们在138个回交到F344菌株的后代中测试了它们的影响，这些后代接受了10周的慢性雌激素治疗。血红蛋白/DNA比（血容量相对于细胞数量的测量）和总垂体DNA（细胞数量的测量）仅弱相关，并且观察到非常大的垂体，其具有类似于正常腺体的低血红蛋白/DNA比。通过QTL定位发现，Edpm 2 -1、Edpm 3、Edpm 5和Edpm 9 - 2对垂体质量均有显著影响，但Edpm 2 - 1和Edpm 9 - 2主要影响DNA含量，Edpm 5主要影响血红蛋白/DNA比值，Edpm 3对所有性状的影响程度相同。
Estrogen treatment to rats of the Fischer 344 (F344) strain induces growth of pituitary tumors that exhibit accelerated cell proliferation, breakdown of basement membrane, and formation of hemorrhagic lakes. Estrogen-dependent pituitary growth is due to variation in a group of quantitative trait loci (QTL), calledEdpmforestrogen-dependentpituitarymass, that we previously identified in an F2intercross of F344 and the tumor-resistant Brown Norway strain. We previously identified 5 QTL, and microsatellite markers developed since our earlier work have allowed us to scan new chromosomal regions, resulting in two new QTL for estrogen-dependent pituitary mass:Edpm9-2and a possible QTL on the X Chromosome (Chr). Here we report evidence that these QTL differ from each other in how they affect growth. To examine the effect of theEdpmQTL on biochemical components of tumor growth, we tested their effects in 138 progeny of a backcross to the F344 strain which were given a 10-week chronic estrogen treatment. Hemoglobin/DNA ratio (a measure of blood volume relative to cell number) and total pituitary DNA (a measure of cell number) correlated only weakly, and very large pituitaries were observed which had a low hemoglobin/DNA ratio resembling a normal gland. Through QTL mapping, we found thatEdpm2-1, Edpm3, Edpm5,andEdpm9-2all had significant effects on pituitary mass, butEdpm2-1andEdpm9-2primarily affected DNA content,Edpm5primarily affected hemoglobin/DNA ratio, andEdpm3affected all traits equally.