IMMUNOHISTOCHEMICAL EVIDENCE FOR APOPTOSIS IN ALZHEIMERS-DISEASE
IMMUNOHISTOCHEMICAL EVIDENCE FOR APOPTOSIS IN ALZHEIMERS-DISEASE
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DOI：
10.1097/00001756-199412000-00031
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发表时间：
1994-12-20
期刊：
影响因子：
1.7
通讯作者：
COTMAN, CW
中科院分区：
文献类型：
--
作者：
SU, JH;ANDERSON, AJ;COTMAN, CW
最近，在我们的实验室和其他人进行的体外研究表明，细胞凋亡可能在阿尔茨海默病（AD）相关的神经元细胞死亡中发挥作用。为了评估这一假设，使用ApopTag系统检查AD、老年对照和手术活检组织的内皮层和内嗅皮层，以检测DNA片段化和DNA染色以揭示核形态。许多神经元细胞核显示不同的形态特征的凋亡存在于缠结轴承神经元以及非缠结轴承神经元在AD脑，而很少或根本没有这样的细胞核中检测到控制脑。我们的体内研究结果支持了这一假设，即细胞凋亡可能是导致AD神经细胞死亡的一种机制。
RECENTLY, in vitro studies conducted in our laboratory and others have suggested that apoptosis may have a role in the neuronal cell death associated with Alzheimer's disease (AD). To evaluate this hypothesis, the hippocampi and entorhinal cortices of AD, aged control, and surgical biopsy tissue were examined using the ApopTag system for the detection of DNA fragmentation and DNA stains to reveal nuclear morphology. Numerous neuronal nuclei displaying distinct morphological characteristics of apoptosis were present within tangle-bearing neurons as well as non-tangle-bearing neurons in AD brain, whereas few or no such nuclei were detected in control brain. Our in vivo results support the hypothesis that apoptosis may be one mechanism leading neuronal cell death in AD.