The Caenorhabditis elegans INX‐4/Innexin is required for the fine‐tuning of temperature orientation in thermotaxis behavior
The Caenorhabditis elegans INX‐4/Innexin is required for the fine‐tuning of temperature orientation in thermotaxis behavior
复制标题
秀丽隐杆线虫 INX-4/Innexin 是趋热行为中温度定向微调所必需的
DOI：
10.1111/gtc.12745
复制
发表时间：
2020
期刊：
影响因子：
2.1
通讯作者：
Mori Ikue
中科院分区：
文献类型：
--
作者：
Tsukamoto Satomi;Emmei Taishi;Nakano Shunji;Nishio Nana;Sasakura Hiroyuki;Mori Ikue
无脊椎动物中的连接蛋白被认为与脊椎动物中的连接蛋白和泛连接蛋白具有相似的作用。然而，它仍然知之甚少，如何innexins功能的生物现象，包括它们在神经系统中的功能。在这里，我们通过对趋热性缺陷突变体的正向筛选，鉴定了秀丽隐杆线虫中的连接蛋白家族成员indix-4。Inx-4突变体在略高于培养温度的温度下表现出异常迁移，称为轻度嗜热行为。拯救实验表明，INX-4在主要的热敏神经元AFD中起作用，以调节趋热行为。INX-4：：GFP融合蛋白仅沿着AFD神经元的轴突定位。此外，INX-4在AFD神经元中的过表达诱导了嗜冷行为，这与INX-4突变体相反。我们的研究结果表明，AFD中的INX-4/Innexin可以在移动到所需温度时微调趋热行为的执行。
Innexins in invertebrates are considered to play roles similar to those of connexins and pannexins in vertebrates. However, it remains poorly understood how innexins function in biological phenomena including their function in the nervous systems. Here, we identifiedinx‐4, a member of the innexin family inC. elegans, by a forward screening of thermotaxis‐defective mutants. Theinx‐4mutants exhibited abnormal migration to a temperature slightly higher than the cultivation temperature, called mild thermophilic behavior. Rescue experiments revealed that INX‐4 acts in the major thermosensory neuron AFD to regulate thermotaxis behavior. INX‐4::GFP fusion protein localized exclusively along axons in AFD neurons. In addition, over‐expression of INX‐4 in AFD neurons induced a cryophilic behavior, which is opposite toinx-4mutants. Our findings suggest that INX‐4/Innexin in AFD may fine‐tune the execution of thermotaxis behavior when moving to desired temperatures.