Retroviruses and mouse embryos: a rapid model for neurovirulence and transplacental antiviral therapy.
Retroviruses and mouse embryos: a rapid model for neurovirulence and transplacental antiviral therapy.
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逆转录病毒和小鼠胚胎：神经毒力和经胎盘抗病毒治疗的快速模型。
DOI：
10.1126/science.3037694
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Ruprecht,RM
中科院分区：
文献类型：
--
作者：
Sharpe,AH;Jaenisch,R;Ruprecht,RM
开发了一种小鼠模型，其中可以在短时间内研究嗜神经性逆转录病毒感染。将Cas-Br-E病毒显微注射到妊娠中期的小鼠胚胎中，在出生后25天内导致瘫痪和死亡，而病毒感染的新生儿仅在4个月后发病。为了评价抗病毒药物是否可以穿过胎盘屏障并影响疾病的进程，通过孕妇的饮用水将药物3′-叠氮基-3 ′-脱氧胸苷（AZT）给予感染的胚胎。叠氮胸苷治疗明显延缓了病毒引起的中枢神经系统疾病的发病和病程，使动物存活超过4个月。这些结果是有效的抗病毒治疗在妊娠期和围产期的证据，并获得性免疫缺陷综合征（艾滋病）病毒的孕产妇传播的管理具有潜在的意义。
A murine model in which neurotropic retroviral infection can be studied over short periods of time was developed. Microinjection of Cas-Br-E virus into midgestation mouse embryos caused paralysis and death within 25 days after birth, in contrast to virus-infected neonates which develop disease only after 4 months. To evaluate whether antiviral drugs could cross the placental barrier and influence the course of the disease, the drug 3′-azido-3′-deoxythymidine (AZT) was administered to infected embryos through the drinking water of pregnant females. AZT treatment markedly retarded the onset and course of virus-induced central nervous system disease, permitting animals to survive beyond 4 months of age. These results are evidence for effective antiviral treatment during gestation and in the perinatal period and are of potential significance for the management of maternal transmission of the acquired immune deficiency syndrome (AIDS) virus.