Influence of collagen and laminin gels concentration on nerve regeneration after resection and tube repair
Influence of collagen and laminin gels concentration on nerve regeneration after resection and tube repair
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DOI：
10.1006/exnr.1997.6650
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发表时间：
1998-01-01
影响因子：
5.3
通讯作者：
Navarro, X
中科院分区：
文献类型：
--
作者：
Labrador, RO;Butí, M;Navarro, X
为了评价胶原蛋白和层粘连蛋白凝胶预充填神经腔促进神经再生的有效性，我们比较了不同组小鼠坐骨神经切除后保留4 mm或6 mm间隙，然后用硅胶管修复后靶器官的再支配情况。用不同浓度的生理盐水、胶原凝胶或含层粘连蛋白的凝胶预充填试管。术后4~5个月，用非侵入性方法评价功能再支配情况，以量化后爪出汗、伤害性感觉、感觉和运动功能的恢复情况。神经残端之间间隙长度的增加推迟了开始时间，降低了功能恢复的程度。用1.28 mg/ml的胶原胶稀释组的再神经再生开始得更早，达到的水平略高于更高浓度(1.92和2.56 mg/ml)的胶原胶，并用生理盐水预填充的管子弥合了4 mm的间隙。稀释液(4 mg/ml)比浓缩层粘连蛋白凝胶(12 mg/ml)的恢复效果更好，但低于胶原凝胶和生理盐水。通过用稀释的胶原蛋白或层粘连蛋白凝胶预填充硅胶管，弥合6 mm的缝隙，这一长度被认为是限制小鼠坐骨神经再生的长度，两种基质都允许比生理盐水溶液更高水平的恢复和成功再生的小鼠。层粘连蛋白凝胶的性能略好于胶原蛋白凝胶。(C)1998年学术出版社。
In order to assess the usefulness of collagen and laminin gels prefilling nerve chambers to enhance nerve regeneration, we compared reinnervation of target organs after sciatic nerve resection leaving gaps of 4 or 6 mm followed by repair with silicone tubes in different groups of mice. Tubes were prefilled with saline solution, collagen gels, or laminin-containing gels at different concentrations. Functional reinnervation was assessed by noninvasive methods to quantitate recovery of sweating, nociceptive, sensory, and motor functions in the hindpaw repeatedly during 4-5 months postoperation. The increase in gap length between nerve stumps delayed the beginning and reduced the degree of functional recovery achieved. Reinnervation started earlier and achieved slightly higher levels with collagen gel diluted at 1.28 mg/ml than with more concentrated (1.92 and 2.56 mg/ml) collagen gels and with saline-prefilled tubes bridging a 4-mm gap. Recovery was also better with diluted (4 mg/ml) than with concentrated (12 mg/ml) laminin-containing gel, although lower than with collagen gels and saline. By prefilling silicone tubes bridging a 6-mm gap, a length considered limiting for regeneration in the mouse sciatic nerve, with diluted collagen or laminin gels, both matrices allowed for higher levels of recovery and for successful regeneration in a higher proportion of mice than saline solution. The laminin gel performed slightly better than the collagen gel. (C) 1998 Academic Press.