Inhaled benzo(a)pyrene impairs long-term potentiation in the F1 generation rat dentate gyrus.
Inhaled benzo(a)pyrene impairs long-term potentiation in the F1 generation rat dentate gyrus.
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发表时间：
2004-09
影响因子：
1.6
通讯作者：
Wormley Dd;S. Chirwa;T. Nayyar;J. Wu;S. Johnson;L. Brown;E. Harris;D. Hood
中科院分区：
文献类型：
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作者：
Wormley Dd;S. Chirwa;T. Nayyar;J. Wu;S. Johnson;L. Brown;E. Harris;D. Hood
本研究的目的是提供一个关于暴露于环境污染物引起的神经毒性效应的参考点。苯并（a）芘是多环芳烃（PAH）家族的一员，它是燃烧过程的副产物。因此，居住在工厂或危险废物场地附近的人面临着通过接触被污染的空气、水和土壤而暴露的危险。为了了解环境污染物的影响，我们研究了妊娠期B（a）P气溶胶暴露对长时程增强（LTP）的影响，LTP是F1代学习和记忆的细胞相关性。简而言之，在妊娠第11-21天，将定时妊娠大鼠通过仅鼻吸入暴露于B（a）P，每天4小时。母鼠饲养至足月，幼仔在出生后第30天断奶。出生后60-70天的后续电生理学研究显示，与未暴露的对照组相比，经胎盘暴露于B（a）P气溶胶的F1代动物海马中贯穿通路-颗粒细胞突触的LTP减少。此外，发现在B（a）P暴露的F1代动物的海马中NMDA受体亚单位1（NR 1）蛋白下调。综上所述，我们的研究结果表明，妊娠期暴露于B（a）P气溶胶减弱了F1代的LTP能力。
The purpose of this study is to provide a point of reference regarding the neurotoxic effects resulting from exposure to environmental contaminants. Benzo(a)pyrene is a member of the polycyclic aromatic hydrocarbon (PAH) family and it is a by-product of combustion processes. Thus, persons living near factories or hazardous waste sites face the danger of exposure through contact with contaminated air, water and soil. In an effort to understand the impact of environmental contaminants, we have investigated the effects of gestational B(a)P aerosol exposure on long-term potentiation (LTP), a cellular correlate of learning and memory in the F1 generation. Briefly, timed-pregnant rats were exposed to B(a)P via nose-only inhalation on gestation days 11-21 for 4 hr per day. Dams were maintained to term and pups were weaned on postnatal day 30. Subsequent electrophysiological studies during postnatal days 60-70 revealed a diminution in LTP across the perforant path-granular cells synapses in the hippocampus of F1 generation animals that were transplacentally exposed to B(a)P aerosol relative to unexposed controls. Additionally, NMDA receptor subunit 1 (NR1) protein was found to be downregulated in the hippocampus of B(a)P exposed F1 generation animals. Taken together, our results suggest that gestational exposure to B(a)P aerosol attenuates the capacity for LTP in the F1 generation.