Discovery of genes with highly restricted expression patterns in the Drosophila wing disc using DNA oligonucleotide microarrays
Discovery of genes with highly restricted expression patterns in the Drosophila wing disc using DNA oligonucleotide microarrays
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DOI：
10.1242/dev.00293
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发表时间：
2003-02-01
期刊：
影响因子：
4.6
通讯作者：
Simcox, A
中科院分区：
文献类型：
--
作者：
Butler, MJ;Jacobsen, TL;Simcox, A
果蝇翅盘沿近端-远端轴沿着分为体壁（近端）、翅铰（中央）和翅叶（远端）。我们应用DNA微阵列分析来发现在这些区域的发展中具有潜在作用的基因。我们确定了一组94个转录丰富（两倍或更多）的体壁和56个转录丰富的翼/铰链区。已知具有高度限制性表达模式的转录本，例如pannier、twist和Bar-H1（体壁）以及knot、nubbin和Distal-less（翼/铰链），在阵列上显示出强烈的差异表达。50个以前未表征的基因的原位杂交同样显示，通过阵列分析鉴定的转录富集与观察到的空间表达一致。有一个广泛的模式，在某些情况下，这表明基因可能是已知的信号通路的目标。我们发现，这些基因中的三个响应无翅信号。我们还发现了可能在气管和成肌细胞类型中发挥特定作用的基因，因为这些细胞是体壁片段的一部分。总之，使用全基因组分析鉴定具有限制性表达模式的基因表明，许多在翅盘发育中具有潜在作用的基因仍有待鉴定。
The Drosophila wing disc is divided along the proximal-distal axis into regions giving rise to the body wall (proximal), wing hinge (central) and wing blade (distal). We applied DNA microarray analysis to discover genes with potential roles in the development of these regions. We identified a set of 94 transcripts enriched (two fold or greater) in the body wall and 56 transcripts enriched in the wing/hinge region. Transcripts that are known to have highly restricted expression patterns, such as pannier, twist and Bar-H1 (body wall) and knot, nubbin and Distal-less (wing/hinge), showed strong differential expression on the arrays. In situ hybridization for 50 previously uncharacterized genes similarly revealed that transcript enrichment identified by the array analysis was consistent with the observed spatial expression. There was a broad spectrum of patterns, in some cases suggesting that the genes could be targets of known signaling pathways. We show that three of these genes respond to wingless signaling. We also discovered genes likely to play specific roles in tracheal and myoblast cell types, as these cells are part of the body wall fragment. In summary, the identification of genes with restricted expression patterns using whole genome profiling suggests that many genes with potential roles in wing disc development remain to be characterized.