Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: Part 1. Structure, gelation rate and mechanical properties
Ionically crosslinked alginate hydrogels as scaffolds for tissue engineering: Part 1. Structure, gelation rate and mechanical properties
复制标题
DOI：
10.1016/s0142-9612(00)00201-5
复制
发表时间：
2001-03-01
期刊：
影响因子：
14
通讯作者：
Ma, PX
中科院分区：
文献类型：
--
作者：
Kuo, CK;Ma, PX
藻酸盐凝胶已以珠粒形式用于药物递送和细胞封装应用，通常通过将藻酸盐溶液滴入CaCl 2浴中来生产。这些系统的主要缺点是凝胶化速率难以控制，所得结构不均匀，并且难以实现机械强度和复杂形状的3-D结构。在这项工作中，控制凝胶化速率实现与CaCO 3-GDL和CaSO 4-CaCO 3-GDL系统，和均匀的藻酸盐凝胶配制成具有定义的尺寸的支架用于组织工程应用。随着总钙含量的增加、CaSO_4比例的增加、温度的升高和海藻酸钠浓度的降低，海藻酸钠的产率增加。藻酸盐凝胶的机械性能由组成变量控制。较慢的凝胶化系统比较快的凝胶化系统产生更均匀和机械强度更强的凝胶。抗压模量和抗压强度随海藻酸钠浓度、海藻酸钠中总钙含量、海藻酸钠分子量和古罗糖醛酸含量的增加而增加。将MC 3 T3-E1成骨细胞均匀地掺入藻酸盐凝胶中并进行体外培养。这些结果证明了藻酸盐凝胶和凝胶/细胞系统可以如何以受控的结构配制。凝胶化速率和用于组织工程和其它生物医学应用的机械性能。(C)2001爱思唯尔科技有限公司版权所有。
Alginate gels have been used in both drug delivery and cell encapsulation applications in the bead form usually produced by dripping alginate solution into a CaCl2 bath. The major disadvantages to these systems are that the gelation rate is hard to control, the resulting structure is not uniform, and mechanically strong and complex-shaped 3-D structures are difficult to achieve. In this work controlled gelation rate was achieved with CaCO3-GDL and CaSO4-CaCO3-GDL systems, and homogeneous alginate gels were formulated as scaffolds with defined dimensions for tissue engineering applications. Gelation rate increased with increasing total calcium content, increasing proportion of CaSO4, increasing temperature and decreasing alginate concentration. Mechanical properties of the alginate gels were controlled by the compositional variables. Slower gelation systems generate more uniform and mechanically stronger gels than faster gelation systems. The compressive modulus and strength increased with alginate concentration, total calcium content, molecular weight and guluronic acid (G) content of the alginate. MC3T3-E1 osteoblastic cells were uniformly incorporated in the alginate gels and cultured in vitro. These results demonstrated how alginate gel and gel/cell systems could be Formulated with controlled structure. gelation rate, and mechanical properties for tissue engineering and other biomedical applications. (C) 2001 Elsevier Science Ltd. All rights reserved.