Development of a smart activity-based probe to detect subcellular activity of asparaginyl endopeptidase in living cells
Development of a smart activity-based probe to detect subcellular activity of asparaginyl endopeptidase in living cells
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DOI：
10.1039/c7ob01467h
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发表时间：
2017-10-14
影响因子：
3.2
通讯作者：
Lee, Jiyoun
中科院分区：
文献类型：
--
作者：
Hong, Jong-Ah;Choi, Na-Eun;Lee, Jiyoun
我们开发了一种基于活性的智能探针，可以检测活细胞中天冬酰胺酰内肽酶（AEP）的活性，以监测酶调节的动态。新设计的探针响应于活细胞中AEP的活性而产生开启荧光信号，而不影响标记效率或选择性。我们的探针密切反映了酶在其天然状态下的活性，检测结肠癌细胞和神经元细胞中的亚细胞AEP活性。
We developed a smart activity-based probe that detects the activity of asparaginyl endopeptidase (AEP) in live cells to monitor the dynamics of enzyme regulation. The newly designed probe generated a turn-on fluorescence signal in response to the activity of AEP in living cells without compromising the labelling efficiency or selectivity. Our probe closely reflected the enzyme activity in its native state, detecting subcellular AEP activity in colon cancer cells and neuronal cells.