Nucleotide sequence analysis of the Newcastle disease virus nucleocapsid protein gene and phylogenetic relationships among the Paramyxoviridae
Nucleotide sequence analysis of the Newcastle disease virus nucleocapsid protein gene and phylogenetic relationships among the Paramyxoviridae
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DOI：
10.1016/s0168-1702(01)00427-0
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发表时间：
2002-02-26
期刊：
影响因子：
5
通讯作者：
King, DJ
中科院分区：
文献类型：
--
作者：
Seal, BS;Crawford, JM;King, DJ
对24株新城疫病毒（NDV）的核衣壳蛋白(N)基因进行了克隆和测序。N蛋白残基401的N端区域在分离株中高度保守，发生了一些与系统发育关系相关的保守取代。在c端部分检测到N蛋白的变异性，这与报道的副粘病毒科其他成员相似。以前在其他副粘病毒的N蛋白中鉴定为不变或高度保守的氨基酸也存在于NDV蛋白中。对NDV分离株N基因编码序列的系统发育分析再次证实存在两大类群。一个分支包含的病毒包括1970年之前在美国分离的疫苗和毒株，而第二个分支包括世界各地分离的疫苗和毒株。副粘病毒科成员之间N蛋白氨基酸序列的比较导致NDV和禽副粘病毒6分离为一个不同于风疹病毒属的聚类。这进一步支持了禽副粘病毒在副粘病毒科中被视为单独属的观点。(C) 2002 Elsevier Science B.V.版权所有
The nucleocapsid (N) protein genes from 24 Newcastle disease virus (NDV) isolates representing various pathotypes with different geographical and chronological origins were cloned and sequenced. The N-terminal region of the N protein to residue 401 was highly conserved among isolates with several conservative substitutions occurring that correlated with phylogenetic relationships. Variability of the N protein was detected in the C-terminal portion similar to what has been reported for other members of the Paramyxovirinae. Amino acids previously identified as invariant or highly conserved in N proteins of other paramyxoviruses were also present in the NDV protein. Phylogenetic analysis of N gene coding sequences among NDV isolates again demonstrated the existence of two major groups. One clade contained viruses that included vaccine and virulent strains isolated in the USA prior to 1970 while a second clade included vaccine and virulent viruses isolated worldwide. Comparison of N protein amino acid sequences among members of the Paramyxoviridae resulted in NDV and avian paramyxovirus 6 separating as a cluster distinct from the Rubulavirus genus. This provides further support for avian paramyxoviruses being considered for their own genus among the Paramyxovirinae. (C) 2002 Elsevier Science B.V. All rights reserved.