HUMAN LIPOPROTEIN-LIPASE COMPLEMENTARY-DNA SEQUENCE
HUMAN LIPOPROTEIN-LIPASE COMPLEMENTARY-DNA SEQUENCE
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DOI：
10.1126/science.3823907
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发表时间：
1987-03-27
期刊：
影响因子：
56.9
通讯作者：
LAWN, RM
中科院分区：
文献类型：
--
作者：
WION, KL;KIRCHGESSNER, TG;LAWN, RM
脂蛋白脂肪酶是脂质代谢的关键酶，其作用是水解甘油三酯，为细胞提供游离脂肪酸并影响循环脂蛋白的成熟。已经提出，该酶在肥胖和动脉粥样硬化的发展中起作用。这种人类酶很难纯化，其蛋白质序列迄今尚未确定。编码448个氨基酸的成熟蛋白质的人脂蛋白脂肪酶的互补DNA现已被克隆和测序。序列分析表明，人类脂蛋白脂肪酶，肝脂肪酶和胰脂肪酶是一个基因家族的成员。在几种不同的组织中检测到两种不同种类的脂蛋白脂肪酶信使RNA，它们来自3“-末端多聚腺苷酸化的替代位点。
Lipoprotein lipase is a key enzyme of lipid metabolism that acts to hydrolyze triglycerides, providing free fatty acids for cells and affecting the maturation of circulating lipoproteins. It has been proposed that the enzyme plays a role in the development of obesity and atherosclerosis. The human enzyme has been difficult to purify and its protein sequence was heretofore undetermined. A complementary DNA for human lipoprotein lipase that codes for a mature protein of 448 amino acids has now been cloned and sequenced. Analysis of the sequence indicates that human lipoprotein lipase, hepatic lipase, and pancreatic lipase are members of a gene family. Two distinct species of lipoprotein lipase messenger RNA that arise from alternative sites of 3''-terminal polyadenylation were detected in several different tissues.