COAGGREGATION OF STREPTOCOCCUS-SANGUIS AND OTHER STREPTOCOCCI WITH CANDIDA-ALBICANS
COAGGREGATION OF STREPTOCOCCUS-SANGUIS AND OTHER STREPTOCOCCI WITH CANDIDA-ALBICANS
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DOI：
10.1128/iai.58.5.1429-1436.1990
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发表时间：
1990-05-01
影响因子：
3.1
通讯作者：
SHEPHERD, MG
中科院分区：
文献类型：
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作者：
JENKINSON, HF;LALA, HC;SHEPHERD, MG
测试了13株草绿色组链球菌和2株其它链球菌与白色念珠菌的共聚集，血链球菌菌株通常表现出低水平的粘附至28 °。C-生长指数期酵母细胞，但在37 ℃下酵母细胞对葡萄糖的饥饿。C（或在28 ℃下）C）使它们与这些链球菌的共聚集活性增加至少十倍。这是四个C共有的属性。白色念珠菌菌株，其中两种能够形成菌丝体（6406和MEN），两种不能形成菌丝体（MM 2002和CA 2）。酵母细胞饥饿过程中的共聚集粘附的表达被添加的海藻酸钠或阿替霉素B抑制。S.血链球菌、戈登链球菌和口腔链球菌的共聚集活性测试涵盖了不同的生理和形态类型，但都表现出与饥饿C.白色念珠菌细胞有没有相关的细胞表面疏水性，无论是酵母菌或链球菌细胞，与他们的能力，共聚集。咽峡炎链球菌菌株也与饥饿的酵母细胞共聚集;唾液链球菌和酿脓链球菌与C. albicans的共聚集性;化脓性链球菌没有促进酵母细胞饥饿;变形链球菌和粪肠球菌没有与酵母共聚集。研究了S. sanguis和S. gordonii和C. EDTA和酵母细胞的热或蛋白酶处理可抑制白色念珠菌，而加入乳糖或其它单糖则不可逆。这些观察结果扩展了口腔微生物之间已知的属间共聚集的范围，并表明C。带有草绿色链球菌的白色念珠菌可能对酵母菌在口腔表面的定植很重要。
Thirteen strains of viridans group streptococci and two strains of other streptococci were tested for coaggregation with Candida albicans, Streptococcus sanguis strains generally exhibited low levels of adherence to 28.degree. C-grown exponential-phase yeast cells, but starvation of yeast cells for glucose at 37.degree. C (or at 28.degree. C) increased their coaggregating activity with these streptococci by at least tenfold. This was a property common to four C. albicans strains tested, two of which were able to form mycelia (6406 and MEN) and two of which were not (MM2002 and CA2). The expression of the coaggregation adhesion during yeast cell starvation was inhibited by addition of trichodermia or amphotericin B. The strains of S. sanguis, Streptococcus gordonii, and Streptococcus oralis tested for coaggregating activity encompassed a diverse range of physiological and morphological types, yet all exhibited saturable coaggregation with starved C. albicans cells. There was no correlation of cell surface hydrophobicity, of either yeast or streptococcal cells, with their abilities to coaggregate. Strains of Streptococcus anginosus also coaggregated with starved yeast cells; Streptococcus salivarius and Streptococcus pyogenes coaggregated to a lesser degree with C. albicans, and the coaggregation with S. pyogenes was not promoted by yeast cell starvation; Streptococcus mutans and Enterococcus faecalis did not coaggregate with yeast. The coaggregation reactions of S. sanguis and S. gordonii with C. albicans were inhibited by EDTA and by heat or protease treatment of the yeast cells and were not reversible by the addition of lactose or other simple sugars. These observations extend the range of intergeneric coaggregations that are known to occur between oral microbes and suggest that coaggregations of C. albicans with viridans group streptococci may be important for colonization of oral surfaces by the yeast.