Magnetic nanoparticle clusters radiosensitise human nasopharyngeal and lung cancer cells after alternating magnetic field treatment
Magnetic nanoparticle clusters radiosensitise human nasopharyngeal and lung cancer cells after alternating magnetic field treatment
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DOI：
10.3109/02656736.2015.1063168
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发表时间：
2015-10-03
影响因子：
3.1
通讯作者：
Wu, Gang
中科院分区：
文献类型：
--
作者：
Ma, Jia;Zhang, Zhiping;Wu, Gang
目的：磁性纳米粒子团簇（MNCs）在交变磁场（AMF）中产生的热量可用于热疗癌症治疗。在这里，我们已经根据先前的报道合成了聚丙烯酸包覆的MNCs，具有增加悬浮液中颗粒稳定性的能力。材料和方法：采用MTT法、流式细胞术、克隆形成实验、Western blotting、α-H_2AX实验等方法，研究AMF对MNCs的放射增敏作用及其生物相容性。结果：不同浓度AMF（200 μ g/mL ~ 2 mg/mL）的MNC液温度迅速升高，随着AMF浓度的增加，MNC液温度升高的幅度逐渐增大，随着AMF浓度的增加，MNC液温度升高的幅度逐渐增大。MNCs被细胞内吞，并被发现是生物相容的。发现Hsp 70和caspase-3在AMF、辐射和两者联合处理下对MNCs上调。AMF下的MNCs有效地放射增敏CNE-2细胞和NCI-H460细胞。最后，在AMF和辐射下用MNCs治疗后的肿瘤抑制率显著高于单独治疗后的肿瘤抑制率。放射增敏的机制涉及DNA修复的抑制和诱导apoptosis.Conclusions：在AMF下的MNC液增强了肿瘤细胞在体外和体内的放射敏感性。
Purpose: Heat generated by magnetic nanoparticle clusters (MNCs) in an alternating magnetic field (AMF) can be used for hyperthermia cancer treatment. Here, we have synthesised polyacrylic acid-coated MNCs according to previous report, with the ability to increase particle stability in suspension. Radiosensitisation effects of the MNCs under an AMF were investigated in vitro and in vivo.Materials and methods: MTT assay, flow cytometry, clone formation assay, Western blotting, and a -H2AX experiment were used to explore the biocompatibility and radiosensitisation effect of the MNCs and their putative radiosensitisation mechanism. An NCI-H460 mouse xenograft model was used to investigate the anti-tumour effect under an AMF in vivo.Results: The temperature of MNC fluids at different concentrations (200g/mL to 2mg/mL) increased rapidly. The MNCs were endocytosed by the cells and were found to be biocompatible. Hsp70 and caspase-3 were found to be up-regulated upon MNCs under an AMF, radiation, and combination of both treatments. MNCs under an AMF efficiently radiosensitised both CNE-2 cells and NCI-H460 cells. Finally, the tumour inhibition rate after treatment with MNCs under an AMF and radiation was significantly higher than that after either treatment alone. The mechanism of radiosensitisation putatively involves inhibition of DNA repair and induction of apoptosis.Conclusions: The MNC fluids under an AMF enhanced the radiosensitivity of tumour cells both in vitro and in vivo.