Preparation of a cellulose acetate/organic montmorillonite composite porous ultrafine fiber membrane for enzyme immobilization
Preparation of a cellulose acetate/organic montmorillonite composite porous ultrafine fiber membrane for enzyme immobilization
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DOI：
10.1002/app.43818
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发表时间：
2016-09
影响因子：
3
通讯作者：
Jinning Zhang;Mingyu Song;Xiaoyu Wang;Jieru Wu;Zhanping Yang;Jianhua Cao;Yun Chen;Q. Wei
中科院分区：
文献类型：
--
作者：
Jinning Zhang;Mingyu Song;Xiaoyu Wang;Jieru Wu;Zhanping Yang;Jianhua Cao;Yun Chen;Q. Wei
以醋酸纤维素（CA）为原料，采用静电纺丝法制备了无孔CA膜、无孔CA/有机蒙脱土（O-MMT）膜、多孔CA膜和多孔CA/O-MMT膜4种纤维膜，并将其用于酶的固定化。采用扫描电子显微镜和透射电子显微镜对复合纤维膜的表面形态进行了研究。固定化酶的最适pH为3.5，最适温度为50 °C。与游离酶相比，固定化酶对pH和温度的变化具有更好的稳定性。此外，O-MMT的添加和纤维上的孔改善了储存稳定性和操作稳定性。在四种纤维膜中，多孔纤维CA/O-MMT膜对固定化酶的稳定性最好。© 2016 Wiley Periodicals，Inc. J.应用聚合物Sci.2016，133，43818.
Four types of fibrous membranes based on cellulose acetate (CA)—CA membranes with nonporous fibers, CA/organic montmorillonite (O‐MMT) membranes with nonporous fibers, CA membranes with porous fibers, and CA/O‐MMT membranes with porous fibers—were prepared by electrospinning, and then, they were used for enzyme immobilization. The surface morphologies of the composite fibrous membranes were investigated with scanning electron microscopy and transmission electron microscopy. The optimum pH was 3.5 for all of the immobilized enzymes, and the optimum temperature was 50 °C. Compared with the free enzyme, the immobilized enzyme showed better stability for pH and temperature changes. Moreover, the addition of O‐MMT and the pores on the fibers improved the storage stability and the operational stability. Among the four kinds of fibrous membranes, the CA/O‐MMT membranes with porous fibers showed the best stability for the immobilized enzymes. © 2016 Wiley Periodicals, Inc. J. Appl. Polym. Sci.2016,133, 43818.