Glucosylglycerol, a compatible solute, sustains cell division under salt stress
Glucosylglycerol, a compatible solute, sustains cell division under salt stress
复制标题
DOI：
10.1104/pp.102.017277
复制
发表时间：
2003-04-01
期刊：
影响因子：
7.4
通讯作者：
Murata, N
中科院分区：
文献类型：
--
作者：
Ferjani, A;Mustardy, L;Murata, N
蓝细菌集胞藻6803在盐胁迫下积累相容性溶质葡萄糖基甘油（GG）和蔗糖。虽然GG合成的分子机制，包括GG-磷酸合酶（ggpS）基因，编码GgpS的调控，已被深入研究，GG在保护盐胁迫的作用仍然知之甚少。在我们对GG在盐胁迫耐受性中的作用的研究中，我们发现由于450 mm NaCl引起的盐胁迫抑制DeltaggpS突变体细胞的细胞分裂并显著增加细胞大小，而在高浓度NaCl（例如800 mm）下在野生型细胞中观察到细胞分裂的抑制和细胞大小的增加。电子显微镜显示，在DeltaggpS细胞中，子细胞的分离是不完全的，并且可以通过类似于分裂环的结构的存在来识别中止的分裂。在培养基中添加GG可保护DeltaggpS细胞免受盐胁迫，并逆转NaCl对细胞分裂和细胞大小的不利影响。这些观察结果表明，GG是重要的耐盐性，从而在盐胁迫条件下的细胞的适当分裂。
The cyanobacterium Synechocystis sp. PCC 6803 accumulates the compatible solute glucosylglycerol (GG) and sucrose under salt stress. Although the molecular mechanisms for GG synthesis including regulation of the GG-phosphate synthase (ggpS) gene, which encodes GgpS, has been intensively investigated, the role of GG in protection against salt stress remains poorly understood. In our study of the role of GG in the tolerance to salt stress, we found that salt stress due to 450 mm NaCl inhibited cell division and significantly increased cell size in DeltaggpS mutant cells, whereas the inhibition of cell division and increase in cell size were observed in wild-type cells at high concentrations of NaCl, such as 800 mm. Electron microscopy revealed that, in DeltaggpS cells, separation of daughter cells was incomplete, and aborted division could be recognized by the presence of a structure that resembled a division ring. The addition of GG to the culture medium protected DeltaggpS cells against salt stress and reversed the adverse effects of NaCl on cell division and cell size. These observations suggest that GG is important for salt tolerance and thus for the proper division of cells under salt stress conditions.