Effects of growth factors on temporomandibular joint disc cells
Effects of growth factors on temporomandibular joint disc cells
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DOI：
10.1016/j.archoralbio.2004.01.015
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发表时间：
2004-07-01
影响因子：
3
通讯作者：
Athanasiou, KA
中科院分区：
文献类型：
--
作者：
Detamore, MS;Athanasiou, KA
生长因子对软骨组织的作用已被广泛报道。一个例外是颞下颌关节（TMJ）盘，其中生长因子对增殖和生物合成的影响的数据非常有限。本研究的目的是定量的增殖和合成的TMJ椎间盘细胞培养在单层与血小板衍生生长因子-AB（PDGF），碱性成纤维细胞生长因子（bFGF）或胰岛素样生长因子-1（IGF），在低（10 ng/ml）或高（100 ng/mL）浓度。在14天时，用DNA定量技术评估增殖，通过羟脯氨酸测定法测量胶原合成，并用二甲基亚甲蓝染料结合测定法测定GAG合成。总体而言，最有益的生长因子是bFGF，其在增加增殖和GAG合成方面最有效，并且在促进胶原蛋白合成方面也有效。在高浓度下，bFGF导致比对照多96%的细胞，比PDGF和IGF多30%至45%的细胞。PDGF和bFGF是GAG合成的最有效的上调剂，产生比对照多2-3倍的GAG。IGF对GAG产生没有显著影响，尽管在其较高浓度下，其使胶原产生比对照增加4.5倍。胶原蛋白的合成促进bFGF在其较低的浓度，水平比对照组高4.2倍，而PDGF对胶原蛋白的产生没有显着的影响。一般而言，较高浓度增加增殖，而较低浓度有利于生物合成。(C)2004 Elsevier Ltd.保留所有权利。
The effects of growth factors on cartilaginous tissues are welt documented. An exception is the temporomandibular joint (TMJ) disc, where data for growth factor effects on proliferation and biosynthesis are very limited. The purpose of this study was to quantify proliferation of and synthesis by TMJ disc cells cultured in monolayer with either ptatelet derived growth factor-AB (PDGF), basic fibrobtast growth factor (bFGF) or insulin-like growth factor-1 (IGF), at either a low (10 ng/ml) or high (100 ng/mL) concentration. Proliferation was assessed with a DNA quantitation technique, collagen synthesis was measured via a hydroxyproline assay, and GAG synthesis was determined with a dimethylmethylene blue dye binding assay at 14 days. Overall, the most beneficial growth factor was bFGF, which was most potent in increasing proliferation and GAG synthesis, and also effective in promoting collagen synthesis. At the high concentration, bFGF resulted in 96% more cells than the control and 30 to 45% more cells than PDGF and IGF. PDGF and bFGF were the most potent upregulators of GAG synthesis, producing 2-3 times more GAG than the control. IGF had no significant effect on GAG production, although at its higher concentration it increased collagen production by 4.5 times over the control. Collagen synthesis was promoted by bFGF at its lower concentration, with levels 4.2 times higher than the control, whereas PDGF had no significant effect on collagen production. In general, higher concentrations increased proliferation, whereas Lower concentrations favoured biosynthesis. (C) 2004 Elsevier Ltd. All rights reserved.