Evidence for the presence of 5S rRNA in mammalian mitochondria
Evidence for the presence of 5S rRNA in mammalian mitochondria
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DOI：
10.1091/mbc.9.9.2375
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发表时间：
1998-09-01
影响因子：
3.3
通讯作者：
Schon, EA
中科院分区：
文献类型：
--
作者：
Magalhaes, PJ;Andreu, AL;Schon, EA
哺乳动物线粒体核糖体含有两个原核样rRNA，12S和16S，均由线粒体DNA编码。然而，与胞质核糖体相反，这些核糖体被认为不含有5S rRNA。由于这个原因，目前还不清楚是否5S rRNA，这可以在线粒体制剂中检测到，是一个真正的细胞器物种进口的胞质溶胶或仅仅是一个copurifying胞质溶胶衍生的污染物。我们现在表明，5S rRNA与高度纯化的人和大鼠细胞的线粒体组分紧密相关，并且来自瞬时转染到人细胞中的合成基因的5S rRNA转录物在体内表达并存在于高度纯化的线粒体和有丝分裂体中。我们的结论是，5S rRNA输入到哺乳动物线粒体，但其功能仍有待澄清。
Mammalian mitochondrial ribosomes contain two prokaryotic-like rRNAs, 12S and 16S, both encoded by mitochondrial DNA. As opposed to cytosolic ribosomes, however, these ribosomes are not thought to contain 5S rRNA. For this reason, it has been unclear whether 5S rRNA, which can be detected in mitochondrial preparations, is an authentic organellar species imported from the cytosol or is merely a copurifying cytosol-derived contaminant. We now show that 5S rRNA is tightly associated with highly purified mitochondrial fractions of human and rat cells and that 5S rRNA transcripts derived from a synthetic gene transfected transiently into human cells are both expressed in vivo and present in highly purified mitochondria and mitoplasts. We conclude that 5S rRNA is imported into mammalian mitochondria, but its function there still remains to be clarified.