Up-regulated long non-coding RNA H19 contributes to proliferation of gastric cancer cells
Up-regulated long non-coding RNA H19 contributes to proliferation of gastric cancer cells
复制标题
上调长链非编码RNA H19有助于胃癌细胞增殖
DOI：
10.1111/j.1742-4658.2012.08694.x
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发表时间：
2012-09-01
期刊：
影响因子：
5.4
通讯作者：
Fang, Guoen
中科院分区：
文献类型：
--
作者：
Yang, Feng;Bi, Jianwei;Fang, Guoen
长非编码RNA(Long Non-Coding RNAs，LncRNAs)在肿瘤生物学中具有重要的调控作用，而LncRNAH19在低氧应激和某些肿瘤中表达上调。然而，H19在胃癌中的作用在很大程度上仍不清楚。本研究采用实时荧光定量聚合酶链式反应检测H19在胃癌组织中的表达水平，并通过流式细胞仪和RNA免疫沉淀法确定H19的生物学功能。我们发现，与正常对照组相比，胃癌细胞和胃癌组织中H19水平显著升高。此外，H19的异位表达促进了AGS细胞的增殖，而H19 siRNA处理促进了AGS细胞的凋亡。我们进一步验证了H19与P53相关，并且这种关联导致了P53的部分失活。这些数据提示H19在胃癌分子病因学中的重要作用以及H19在胃癌治疗中的潜在应用。
Long non-coding RNAs (lncRNAs) have been shown to have important regulatory roles in cancer biology, and the lncRNA H19 is up-regulated in hypoxic stress and in some tumors. However, the contributions of H19 to gastric cancer remain largely unknown. In this study, we assayed the H19 expression level in gastric cancer tissues by real-time PCR, and defined the biological functions by flow cytometry and RNA immunoprecipitation. We demonstrated that H19 levels were markedly increased in gastric cancer cells and gastric cancer tissues compared with normal controls. Moreover, ectopic expression of H19 increased cell proliferation, whereas H19 siRNA treatment contributed to cell apoptosis in AGS cell line. We further verified that H19 was associated with p53, and that this association resulted in partial p53 inactivation. These data suggest an important role for H19 in the molecular etiology of gastric cancer and potential application of H19 in gastric cancer therapy.