Multidrug resistance-associated protein gene expression and drug sensitivity in human lung cancer cells.
Multidrug resistance-associated protein gene expression and drug sensitivity in human lung cancer cells.
复制标题
人肺癌细胞中多药耐药相关蛋白基因表达和药物敏感性。
作者：
F. Narasaki;M. Oka;M. Fukuda;K. Ikeda;K. Terashi;H. Takatani;T. Nakamura;H. Soda;S. Kohno
检测肺癌细胞中多药耐药相关蛋白（MRP） mRNA的表达和药物敏感性，并检测MRP调节剂维拉帕米对药物敏感性的影响。9个细胞系表达不同水平的MRP基因表达，但不表达MDR1基因。非小细胞癌（NSCLC）的水平高于小细胞癌（SCLC）。MRP基因水平与对依托泊苷（VP-16）和阿霉素（Dox）的敏感性之间存在明显的相关性，但有一个细胞系高度表达DNA拓扑异构酶II。三种细胞系的MRP基因水平与维拉帕米对VP-16、Dox和长春新碱的调节作用呈正相关。目前的结果表明，MRP可能在非小细胞肺癌中而不是在小细胞肺癌中赋予了内在的多药耐药。
Multidrug resistance-associated protein (MRP) mRNA expression and drug sensitivity in lung cancer cells were examined, and the effects of verapamil, a modulating agent for MRP, on drug sensitivity were also tested. Nine cell lines expressed various levels of MRP gene expression but not the MDR1 gene. The levels were higher in non-small cell carcinoma cells (NSCLC) than in small cell carcinoma cells (SCLC). Clear correlations between the MRP gene level and the sensitivity to etoposide (VP-16) and doxorubicin (Dox) were observed except for one cell line which highly expressed DNA topoisomerase II. Positive correlations between the MRP gene levels in three cell lines and the modulation effects of verapamil in VP-16, Dox, and vincristine were observed. The present results indicate that MRP probably confers intrinsic multidrug resistance in NSCLC rather than in SCLC.