Dissecting lipid metabolism in meibomian glands of humans and mice: An integrative study reveals a network of metabolic reactions not duplicated in other tissues.
Dissecting lipid metabolism in meibomian glands of humans and mice: An integrative study reveals a network of metabolic reactions not duplicated in other tissues.
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DOI：
10.1016/j.bbalip.2016.03.024
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发表时间：
2016-06
期刊：
影响因子：
--
通讯作者：
Itani K
中科院分区：
文献类型：
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作者：
Butovich IA;McMahon A;Wojtowicz JC;Lin F;Mancini R;Itani K
脂质包括由睑板细胞产生的睑板腺分泌物（睑脂）的大部分。睑板腺中复杂的脂肪生成反应，我们统称为睑板生成，尚未在分子水平上探索。我们的目标是阐明可能的生物合成途径，下一代的睑脂，揭示的相似性，和差异，在睑板腺和其他器官和组织的脂质代谢，并整合睑板腺的研究领域的一般代谢组学。具体来说，我们已经进行了详细的分析，人类和小鼠标本，使用基因组学，免疫组化和脂质组学的方法。在这两个物种的睑板腺中发现的同样高度表达的基因中，有那些与脂肪酸延伸、分支、去饱和、酯化、脂肪酸还原为醇和胆固醇生物合成相关的基因。重要的是，使用脂质组学方法在两个物种的睑脂中检测到相应的脂质产物。第一次，一个有凝聚力的，统一的生物合成方案，连接基因组，lipidomic，和免疫组织化学观察概述和讨论。
Lipids comprise the bulk of the Meibomian gland secretion (meibum) which is produced by meibocytes. Complex arrays of lipogenic reactions in Meibomian glands, which we collectively call meibogenesis, have not been explored on molecular level yet. Our goals were to elucidate the possible biosynthetic pathways that underlie generation of meibum, reveal similarities in, and differences between, lipid metabolism in Meibomian glands and other organs and tissues, and integrate Meibomian gland studies into the field of general metabolomics. Specifically, we have conducted detailed analyses of human and mouse specimens using genomic, immunohistochemical, and lipidomic approaches. Among equally highly expressed genes found in Meibomian glands of both species were those related to fatty acid elongation, branching, desaturation, esterification, reduction of fatty acids to alcohols, and cholesterol biosynthesis. Importantly, corresponding lipid products were detected in meibum of both species using lipidomic approaches. For the first time, a cohesive, unifying biosynthetic scheme that connects genomic, lipidomic, and immunohistochemical observations is outlined and discussed.