BRAIN ENERGY-METABOLISM OF CONSCIOUS RAT EXPOSED TO VARIOUS PHYSIOLOGICAL AND PATHOLOGICAL SITUATIONS
BRAIN ENERGY-METABOLISM OF CONSCIOUS RAT EXPOSED TO VARIOUS PHYSIOLOGICAL AND PATHOLOGICAL SITUATIONS
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DOI：
10.1016/0006-8993(76)90944-6
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发表时间：
1976-01-01
期刊：
影响因子：
2.9
通讯作者：
MAYEVSKY, A
中科院分区：
文献类型：
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作者：
MAYEVSKY, A
使用分时荧光反射计，吡啶核苷酸（NADH）和黄素蛋白（Fp）的荧光，并在激发波长的反射光，进行了测量和相关的电活动清醒的大脑皮层。大鼠暴露于N2气氛（缺氧）导致吡啶核苷酸和黄素减少的信号增加，具有非常相似的动力学。通过双侧颈动脉结扎诱导部分缺血导致NADH增加，伴随着对电活动（ECoG [皮质电图]）的非常小的影响。大多数动物在结扎后2-3 h，ECoG变得平坦或凹陷。此缺血大脑皮层暴露于KCl溶液引起抑郁症的电活动没有代谢反应可能是由于O2供应的限制。通过将大脑暴露于不同水平的O2、癫痫样活动、扩散性抑郁、O2的高压和解偶联剂来确定清醒大脑皮层的代谢状态。清醒的大脑皮层接近于静息状态，即状态4，而不是活跃状态，即状态3。
Using a time-sharing fluorometer-reflectometer, pyridine nucleotide (NADH) and flavoprotein (Fp) fluorescence, and reflected light at the excitation wavelength, were measured and correlated with the electrical activity of an awake cerebral cortex. Exposure of the rat to a N2 atmosphere (anoxia) led to an increase in signals representing the reduction of pyridine nucleotides and flavins, with very similar kinetics. Inducement of partial ischemia by bilateral carotid artery ligation led to an increase in NADH, accompanied by a very small effect on the electrical activity (ECoG [electrocorticogram]). In most animals, 2-3 h after ligation, the ECoG became flat or depressed. Exposure of this ischemic cerebral cortex to KCl solution caused depression of the electrical activity without metabolic response probably due to the limitation of O2 supply. The metabolic state of an awake cerebral cortex was identified by exposing the brain to various levels of O2, epileptiform activity, spreading depression, hyperbaric pressure of O2 and an uncoupler. The awake cerebral cortex is close to the resting state, state 4, rather than to the active state, state 3.