Thresholds of intracranially recorded auditory field potentials in the pigeon compared with compound action potential thresholds.
Thresholds of intracranially recorded auditory field potentials in the pigeon compared with compound action potential thresholds.
复制标题
鸽子颅内记录的听觉场电位阈值与复合动作电位阈值的比较。
DOI：
10.1159/000259243
复制
发表时间：
1997
影响因子：
1.6
通讯作者：
R. Klinke
中科院分区：
文献类型：
--
作者：
B. Sarkar;I. Stiebler;J. Smolders;R. Klinke
复合动作电位（CAP）阈值提供了一个可靠的指标，耳蜗功能的完整性在实验期间，在鸟类以及在哺乳动物。然而，如果在中耳/内耳空间中需要实验操作，圆窗电极通常是不方便的。为了寻找CAP记录的替代方法，使用立体定位放置的电极在鸽子中进行了角核/大细胞核的声诱发场电位的颅内记录。将这些反应与放置在硬脑膜上的颅内表面电极记录的反应进行比较，并与圆窗记录的CAP反应进行比较。从角状核/大细胞核记录的场电位包含来自听神经的显著贡献，其幅度与在圆窗处记录的CAP一样大。来自颅内表面电极的记录噪音较大，并且来自听神经的贡献太小，不能用作内耳状况的快速监测器。阈值曲线作为频率的函数可以用自动化方法从角状核/大细胞核中确定，其灵敏度和准确度与圆窗CAP相同，在几分钟内即可确定。这些结果表明，从核magnocellularis/angularis区域的场电位的立体定向记录是一个合适的替代可靠地监测内耳的条件时，圆窗电极不能使用。
The compound action potential (CAP) thresholds provide a reliable indicator for cochlear functional integrity during experimentation in birds as well as in mammals. However, if experimental manipulations are necessary in the middle ear/inner ear spaces, the round window electrodes are often inconvenient. In search for an alternative for CAP recordings, intracranial recordings of acoustically evoked field potentials from the nucleus angularis/magnocellularis were made in pigeons using stereotactically placed electrodes. The responses were compared with those recorded from intracranial surface electrodes placed on the dura mater and compared with CAP responses recorded from the round window. The field potentials recorded from the nucleus angularis/magnocellularis contain a significant contribution from the auditory nerve, as large in amplitude as the CAP recorded at the round window. The recordings from the intracranial surface electrodes were noisier and the contribution from the auditory nerve was too small to be used as a fast monitor of the condition of the inner ear. Threshold curves as a function of frequency could be determined with an automated method from the nucleus angularis/magnocellularis with the same sensitivity and accuracy as from the round window CAP within a few minutes. These results demonstrate that stereotactic recordings of field potentials from the nucleus magnocellularis/angularis region are a suitable alternative to reliably monitor the condition of the inner ear when round window electrodes cannot be used.