A novel strategy to reveal the latent abnormalities in human embryonic stages from a large embryo collection
A novel strategy to reveal the latent abnormalities in human embryonic stages from a large embryo collection
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从大量胚胎中揭示人类胚胎阶段潜在异常的新策略
DOI：
10.1002/ar.23281
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Takakuwa T
中科院分区：
文献类型：
--
作者：
Kanahashi T;Yamada S;Tanaka M;Hirose A;Uwabe C;Kose K;Yoneyama A;Takeda T;Takakuwa T
在大多数情况下，正常妊娠自然流产的原因尚不清楚。该研究旨在通过使用来自磁共振成像（MRI）数据库和新型相对比放射计算机断层扫描（PXCT）的大量胚胎图像来揭示潜在的异常。以肝脏体积为靶器官，对来自京都收集的1156例卡内基期（CS） 14至CS23之间的胚胎进行MRI筛选。筛选肝脏体积≥2 SD高于或低于发育阶段平均水平的胚胎，并在MRI上进行精确检查。对肝脏可能异常的胚胎进行PXCT进一步分析。特小肝组的7个胚胎均有肝脏异常，特大肝组的8个胚胎中有2个出现肝脏异常。特小肝组的异常包括肝发育不全（2个胚胎）、肝发育不全（4个）和肝叶缺损（1个）。在7例特小肝组中，2例仅出现肝脏异常，5例出现其他器官并发症。超大肝脏组的2个胚胎中，一个只有一个肝脏异常，另一个肝脏形态异常并伴有其他器官并发症。由于肝功能的需要，这种肝功能异常的病例大多不能存活。在体外正常胚胎的宫内群体中，CS18和CS21的肝脏畸形患病率约为1.7%。本研究是阐明外部正常胚胎中导致自然流产的潜在异常的第一步。中国生物医学工程学报，2016,29(9):829 - 829。©2015 Wiley期刊公司
The cause of spontaneous abortion of normal conceptuses remains unknown in most cases. The study was aimed to reveal the latent abnormalities by using a large collection of embryo images from a magnetic resonance imaging (MRI) database and novel phase‐contrast radiographic computed tomography (PXCT). MRI from 1,156 embryos between Carnegie stage (CS) 14 and CS23 from the Kyoto Collection were screened by using the volume of the liver as the target organ. Embryos with liver volumes ≥2 SD above or below the mean for the stage of development were screened and examined precisely on MRI. Embryos with potentially abnormal livers were further analyzed by using PXCT. Liver abnormality was detected in all 7 embryos in the extra‐small liver group and in 2 of 8 embryos in the extra‐large liver group. The abnormalities in the extra‐small liver group consisted of hepatic agenesis (2 embryos), hepatic hypogenesis (4), and liver lobe defect (1). Among the 7 extra‐small liver group, 2 had only liver abnormalities and 5 exhibited complications in other organs. Of the 2 embryos in the extra‐large liver group, one had only a single liver abnormality and the other had a morphologically abnormal liver with complications in other organs. Most of such liver abnormality cases are not survive, as liver function becomes essential. The prevalence of liver malformations in CS18 and CS21 in the intrauterine population of externally normal embryos is approximately 1.7%. The present study is the first step toward the elucidation of the latent abnormalities resulting in spontaneous abortion in externally normal embryos. Anat Rec, 299:8–24, 2016. © 2015 Wiley Periodicals, Inc.