Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo
Genome-wide expression profiling reveals distinct clusters of transcriptional regulation during bovine preimplantation development in vivo
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DOI：
10.1073/pnas.0805616105
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发表时间：
2008-12-16
影响因子：
11.1
通讯作者：
Niemann, H.
中科院分区：
文献类型：
--
作者：
Kues, W. A.;Sudheer, S.;Niemann, H.
牛胚胎可以通过体外受精或体细胞核移植产生，但这些胚胎在许多方面与体内胚胎不同，并显示出更高比例的发育异常。在这里，我们使用Affymetrix基因芯片牛基因组阵列首次测定了牛中期II卵母细胞和体内发育到囊胚的植入前胚胎的所有阶段的转录本，该阵列检查了大约23,000个转录本。数据显示，牛卵母细胞和胚胎转录的基因数量明显高于体细胞。数百个基因在8-细胞阶段之前就被转录了，在这个阶段，牛基因组表达的主要激活发生了。重要的是，在2-细胞、4-细胞和8-细胞阶段，以及在桑椹胚和囊胚中，检测到了特定阶段的表达模式，表明了胚胎转录组和一组瞬时活性基因的动态变化。通路分析显示>；120个生化通路在牛早期植入前发育中起作用。在体内成熟的卵母细胞和体外成熟的卵母细胞的mRNA表达谱之间观察到了显著的差异，这突显了在评估辅助生殖技术的研究中包括体内来源的卵母细胞/胚胎的必要性。本研究首次全面分析了牛胚胎在体内发育过程中的基因表达和转录组动态变化，为进一步完善辅助生殖技术奠定了基础。
Bovine embryos can be generated by in vitro fertilization or somatic nuclear transfer; however, these differ from their in vivo counterparts in many aspects and exhibit a higher proportion of developmental abnormalities. Here, we determined for the first time the transcriptomes of bovine metaphase II oocytes and all stages of preimplantation embryos developing in vivo up to the blastocyst using the Affymetrix GeneChip Bovine Genome Array which examines approximately 23,000 transcripts. The data show that bovine oocytes and embryos transcribed a significantly higher number of genes than somatic cells. Several hundred genes were transcribed well before the 8-cell stage, at which the major activation of the bovine genome expression occurs. Importantly, stage-specific expression patterns in 2-cell, 4-cell, and 8-cell stages, and in morulae and blastocysts, were detected, indicating dynamic changes in the embryonic transcriptome and in groups of transiently active genes. Pathway analysis revealed > 120 biochemical pathways that are operative in early preimplantation bovine development. Significant differences were observed between the mRNA expression profiles of in vivo and in vitro matured oocytes, highlighting the need to include in vivo derived oocytes/embryos in studies evaluating assisted reproductive techniques. This study provides the first comprehensive analysis of gene expression and transcriptome dynamics of in vivo developing bovine embryos and will serve as a basis for improving assisted reproductive technology.