General primer‐mediated polymerase chain reaction permits the detection of sequenced and still unsequenced human papillomavirus genotypes in cervical scrapes and carcinomas
General primer‐mediated polymerase chain reaction permits the detection of sequenced and still unsequenced human papillomavirus genotypes in cervical scrapes and carcinomas
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DOI：
10.1002/ijc.2910450412
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发表时间：
1990-04
影响因子：
6.4
通讯作者：
A. V. D. van den Brule;P. Snijders;R. Gordijn;O. Bleker;C. Meijer;J. Walboomers
中科院分区：
文献类型：
--
作者：
A. V. D. van den Brule;P. Snijders;R. Gordijn;O. Bleker;C. Meijer;J. Walboomers
新开发的通用引物介导的聚合酶链反应（GP-PCR）用于检测细胞学正常和异常宫颈涂片以及宫颈癌活检中的广谱人乳头瘤病毒（HPV）基因型，包括未测序的类型。该PCR方法使用不同的通用引物组，位于HPV基因组的高度保守的E1和L1区域。GP-PCR和HPV-型特异性PCR（TS-PCR）结果之间的比较显示，在正常、轻度和重度异型增生细胞的刮擦中，总体HPV患病率分别增加至25%、80%和88%。在11%的细胞学正常拭子和高达30%的具有异型增生细胞的刮擦中检测到未测序的HPV类型。进一步的表征表明，未测序的类型涉及HPV 13，30，31，45，51和其他一些可能未知的HPV类型。超过90%的原位癌和浸润性宫颈癌含有HPV。在后者中，仅存在HPV 16和HPV 18。HPV 16在正常细胞和异型增生细胞中的检出率最高，在肿瘤组织中的检出率最高。这些结果表明，GP-PCR是检测与宫颈鳞状细胞上皮肿瘤转化相关的尚未表征的HPV类型的有效方法。
A newly developed general primer‐mediated polymerase chain reaction (GP‐PCR) was used for the detection of a broad spectrum of Human Papilloma‐virus (HPV) genotypes, including unsequenced types, in cytologically normal and abnormal cervical smears and in biopsies of cervical carcinomas. This PCR method used different general primer sets, located in strongly conserved E1 and L1 regions of the HPV genome. Comparison between results of GP‐PCR and HPV‐type‐specific PCR (TS‐PCR) revealed an increase in overall HPV prevalence to 25%, 80% and 88% in scrapes with normally, slightly and severely dysplastic cells, respectively. Unsequenced HPV types were detected in 11% of cytologically normal swabs and in up to 30% of scrapes with dysplastic cells. Further characterization showed that unsequenced types concern HPV 13, 30, 31, 45, 51 and some other, possibly unknown HPV types. More than 90% of carcinomas in situ and invasive cervical carcinomas contained HPV. In the latter, only HPV16 and HPV18 were present. HPV16 was most frequently found in both normal and dysplastic cells, the rate being highest in neoplastic tissue. These results indicate that GP‐PCR is a powerful approach for detecting as yet uncharacterized HPV types associated with neoplastic transformation of cervical squamous cell epithelium.