Highly Variable Immune-Response Proteins (185/333) from the Sea Urchin, Strongylocentrotus purpuratus: Proteomic Analysis Identifies Diversity within and between Individuals
Highly Variable Immune-Response Proteins (185/333) from the Sea Urchin, Strongylocentrotus purpuratus: Proteomic Analysis Identifies Diversity within and between Individuals
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DOI：
10.4049/jimmunol.07012766
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发表时间：
2009-02-15
影响因子：
4.4
通讯作者：
Raftos, David A.
中科院分区：
文献类型：
--
作者：
Dheilly, Nowenn M.;Nair, Sham V.;Raftos, David A.
185/333基因和成绩单从紫色海胆，球海胆，预测高水平的氨基酸多样性内编码的蛋白质。基于它们的表达模式，185/333蛋白质似乎参与免疫应答。在本研究中，一维和二维蛋白质印迹显示，185/333蛋白质表现出高水平的分子多样性内和个体海胆之间。185/333阳性条带或斑点的分子量范围为30至250 kDa，具有广泛的等电点。观察到的分子量高于从mRNA预测的分子量，表明185/333蛋白质与其他分子或彼此形成强关联。一些海胆表达了超过200种不同的185/333蛋白质，每种动物都有一套独特的蛋白质，与所有其他个体不同。当海胆在体内与病原体相关的分子模式（PAMPs，细菌LPS和肽聚糖）的挑战，185/333蛋白质的表达增加。更重要的是，不同的185/333蛋白质组响应于不同的PAMP而表达。这表明185/333蛋白的表达可以针对不同的PAMP以病原体特异性免疫应答的形式进行定制。免疫学杂志，2009，182：2203-2212.
185/333 genes and transcripts from the purple sea urchin, Strongylocentrotus purpuratus, predict high levels of amino acid diversity within the encoded proteins. Based on their expression patterns, 185/333 proteins appear to be involved in immune responses. In the present study, one- and two-dimensional Western blots show that 185/333 proteins exhibit high levels of molecular diversity within and between individual sea urchins. The molecular masses of 185/333-positive bands or spots range from 30 to 250 kDa with a broad array of isoelectric points. The observed molecular masses are higher than those predicted from mRNAs, suggesting that 185/333 proteins form strong associations with other molecules or with each other. Some sea urchins expressed > 200 distinct 185/333 proteins, and each animal had a unique suite of the proteins that differed from all other individuals. When sea urchins were challenged in vivo with pathogen-associated molecular patterns (PAMPs; bacterial LPS and peptidoglycan), the expression of 185/333 proteins increased. More importantly, different suites of 185/333 proteins were expressed in response to different PAMPs. This suggests that the expression of 185/333 proteins can be tailored toward different PAMPs in a form of pathogenspecific immune response. The Journal of Immunology, 2009, 182: 2203-2212.