Developmental rate displays effects of inheritance but not of sex in interpopulation hybrids of Tigriopus californicus
Developmental rate displays effects of inheritance but not of sex in interpopulation hybrids of Tigriopus californicus
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DOI：
10.1002/jez.2709
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发表时间：
2023-05-24
影响因子：
2.8
通讯作者：
Burton，Ronald S. S.
中科院分区：
文献类型：
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作者：
Healy，Timothy M. M.;Hargadon，Alexis Cody;Burton，Ronald S. S.
种群内共同进化的遗传相互作用可能会被杂交扰乱，导致杂交个体失去适应度(即杂交崩溃)。然而，目前还不清楚杂交后代在多大程度上遗传了与适合度相关的性状，而且由于雌性和雄性遗传不亲和性的不同影响，这些性状的变异可能是性别特有的。在这里，我们提供了两个实验，研究了潮间足老虎种群间相互杂交的发育速度的差异。在该物种中，发育速度是一个与适合度相关的特征，它受到杂交后代中线粒体编码基因和核编码基因之间的相互作用的影响，从而导致线粒体ATP合成能力的变化。首先，我们证明了在两个正反交组合中，F2代的杂种发育率是相等的，并且不受性别的影响，这表明雌性和雄性可能同样经历着发育率的崩溃。其次，我们证明了F3代发育速率的变异是可遗传的，快速发育的F3亲本的F4代的肋足变态时间(12.25 ± 0.05d，μ ± 扫描电子显微镜)显著快于发育缓慢亲本的F4代(14.58 ± 0.05d)。第三，我们发现这些F4杂交种的ATP合成速率不受其父母发育速率的影响，但雌性线粒体合成ATP的速度快于雄性线粒体。综上所述，这些结果表明，在这些杂交后代中，性别特有的影响在与健康相关的性状中有所不同，而且可能与杂交失败相关的影响在杂交后代中表现出相当大的遗传。
Coevolved genetic interactions within populations can be disrupted by hybridization resulting in loss of fitness in hybrid individuals (i.e., hybrid breakdown). However, the extent to which variation in fitness‐related traits among hybrids is inherited across generations remains unclear, and variation in these traits may be sex‐specific in hybrids due to differential effects of genetic incompatibilities in females and males. Here we present two experiments investigating variation in developmental rate among reciprocal interpopulation hybrids of the intertidal copepodTigriopus californicus. Developmental rate is a fitness‐related trait in this species that is affected by interactions between mitochondrial‐encoded and nuclear‐encoded genes in hybrids that result in variation in mitochondrial ATP synthesis capacities. First, we show that F2‐hybrid developmental rate is equivalent in two reciprocal crosses and is unaffected by sex, suggesting that breakdown of developmental rate is likely experienced equally by females and males. Second, we demonstrate that variation in developmental rate among F3hybrids is heritable; times to copepodid metamorphosis of F4offspring of fast‐developing F3parents (12.25 ± 0.05 days, μ ± SEM) were significantly faster than those of F4offspring of slow‐developing parents (14.58 ± 0.05 days). Third, we find that ATP synthesis rates in these F4hybrids are unaffected by the developmental rates of their parents, but that mitochondria from females synthesize ATP at faster rates than mitochondria from males. Taken together, these results suggest that sex‐specific effects vary among fitness‐related traits in these hybrids, and that effects likely associated with hybrid breakdown display substantial inheritance across hybrid generations.